细胞核中不同磷脂酶C同工型的存在及其在肝脏代偿性生长过程中的激活。
Presence of different phospholipase C isoforms in the nucleus and their activation during compensatory liver growth.
作者信息
Crljen Vladiana, Visnjić Dora, Banfić Hrvoje
机构信息
Department of Physiology and Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Salata 3, 10 000 Zagreb, Croatia.
出版信息
FEBS Lett. 2004 Jul 30;571(1-3):35-42. doi: 10.1016/j.febslet.2004.06.051.
Phospholipase C (PLC) was purified from the membrane-depleted rat liver nuclei. About 60% of the total PLC-activity corresponded to beta1b isoform, 30% to PLC-gamma1 and less than 10% to PLC-delta1. PLC-beta1b and -gamma1 were found in the nuclear matrix, while PLC-delta1 was detected in the chromatin. Two peaks of an increase in the total PLC-activity were detected occurring at 6 and 20 h after partial hepatectomy. An early increase in PLC-beta1b activity in the nuclear matrix was associated with serine phosphorylation of the enzyme, while the later increase paralleled the increase in the amount of protein. The increase in the PLC-gamma1 activity measured at 6 and 20 h after partial hepatectomy was associated with tyrosine phosphorylation of the enzyme. The activity of PLC-delta1 and the amount of the protein found in the chromatin was increased only at 20 h after partial hepatectomy.
磷脂酶C（PLC）是从去除膜的大鼠肝细胞核中纯化得到的。总PLC活性中约60%对应β1b亚型，30%对应PLC-γ1，而对应PLC-δ1的不到10%。在核基质中发现了PLC-β1b和-γ1，而在染色质中检测到了PLC-δ1。在部分肝切除术后6小时和20小时检测到总PLC活性增加的两个峰值。核基质中PLC-β1b活性的早期增加与该酶的丝氨酸磷酸化有关，而后期增加与蛋白质含量的增加平行。部分肝切除术后6小时和20小时测得的PLC-γ1活性增加与该酶的酪氨酸磷酸化有关。仅在部分肝切除术后20小时，染色质中PLC-δ1的活性和蛋白质含量增加。
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