通过定点诱变对Rab偶联蛋白（RCP）的Rab结合特性进行表征。
Characterisation of the Rab binding properties of Rab coupling protein (RCP) by site-directed mutagenesis.
作者信息
Lindsay Andrew J, McCaffrey Mary W
机构信息
Molecular Cell Biology Laboratory, Department of Biochemistry, Biosciences Institute, University College Cork, Cork, Ireland.
出版信息
FEBS Lett. 2004 Jul 30;571(1-3):86-92. doi: 10.1016/j.febslet.2004.06.068.
Rab coupling protein (RCP) is a member of the Rab11-family of interacting proteins (Rab11-FIPs). Family members are characterised by their ability to interact with Rab11. This property is mediated by a conserved Rab binding domain (RBD) located at their carboxy-termini. Several Rab11-FIPs can also interact with other small GTPases. RCP interacts with Rab4 in addition to Rab11. To dissect out the individual properties of the Rab4 and Rab11 interactions with RCP, conserved amino acids within the RBD of RCP were mutated by site-directed mutagenesis. The effect of these mutations on Rab4 and Rab11 binding, and the intracellular localisation of RCP, was examined. Our results indicate that Rab11, rather than Rab4, mediates the intracellular localisation of RCP, and that the class I Rab11-FIPs compete for binding to Rab11.
Rab偶联蛋白（RCP）是Rab11相互作用蛋白家族（Rab11-FIPs）的成员之一。该家族成员的特点是能够与Rab11相互作用。这种特性由位于其羧基末端的保守Rab结合结构域（RBD）介导。几个Rab11-FIPs也能与其他小GTP酶相互作用。除了Rab11，RCP还能与Rab4相互作用。为了剖析Rab4和Rab11与RCP相互作用的各自特性，通过定点诱变对RCP的RBD内的保守氨基酸进行了突变。检测了这些突变对Rab4和Rab11结合以及RCP细胞内定位的影响。我们的结果表明，介导RCP细胞内定位的是Rab11而非Rab4，并且I类Rab11-FIPs竞争与Rab11的结合。
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