Hachey David L, Chaurand Pierre
Department of Pharmacology, Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN 37232, USA.
J Reprod Immunol. 2004 Aug;63(1):61-73. doi: 10.1016/j.jri.2004.01.009.
With the near completion of the human genome project, reproductive biology is poised to enter the vastly more complex arena of proteomics. Proteomics involves the identification, characterization and quantitation of all proteins present in a cell at a particular metabolic state. Although the number of genes in the human genome is estimated to be about 27,000 +/- 5000, the number of proteins produced by humans is unknown, with estimates ranging as high as 1,500,000 distinct molecular entities. In order to address problems in the early stages of reproduction, proteomics must be scaled down to work with very few numbers of cells, termed zeptoproteomics. Mass spectrometry has rapidly become the key technology in proteomics, enabling rapid and facile identification and quantitation of femtomole and attomole quantities of a protein.
随着人类基因组计划即将完成，生殖生物学准备进入更为复杂的蛋白质组学领域。蛋白质组学涉及对处于特定代谢状态的细胞中所有蛋白质的鉴定、表征和定量分析。尽管人类基因组中的基因数量估计约为27000±5000个，但人类产生的蛋白质数量尚不清楚，估计高达150万个不同的分子实体。为了解决生殖早期阶段的问题，蛋白质组学必须缩小规模，以处理极少量的细胞，即zeptoproteomics（zepto为表示10⁻²¹的词头，这里可理解为极微量蛋白质组学）。质谱分析法已迅速成为蛋白质组学中的关键技术，能够快速、便捷地鉴定和定量分析飞摩尔和阿托摩尔数量的蛋白质。
Zotero 插件