[线粒体细胞色素c氧化酶亚基3（cox3）转录本的编辑可能会在野生谷物老芒麦中产生蛋白质-蛋白质相互作用的新位点]

[Editing of the mitochondrial cox3 transcript may yield a new site of protein-protein interactions in wild cereal Elymus sibiricus L].

作者信息

Tauson E L, Verbitskiĭ D S, Klimenko E S, Konstantinov Iu M, Kulinchenko M V, Arziev A Sh, Kobzev V F

出版信息

Mol Biol (Mosk). 2004 May-Jun;38(3):420-8.

Abstract

With PCR, RT-PCR, and direct sequencing, complete nucleotide sequences were established for the Elymus sibiricus mitochondrial cytochrome c oxidase subunit 3 gene (cox3) and its cDNA. The cox3 transcript was shown to have 12 editing sites with changes affecting the amino acid sequence of the protein product. The editing of the primary cox3 transcript was found to change the position of a site of protein-protein interactions. The results demonstrate again the important role of mRNA editing in posttranscriptional regulation of the expression of plant mitochondrial genes.

摘要

通过聚合酶链反应（PCR）、逆转录聚合酶链反应（RT-PCR）和直接测序，确定了老芒麦线粒体细胞色素c氧化酶亚基3基因（cox3）及其cDNA的完整核苷酸序列。结果表明，cox3转录本有12个编辑位点，这些变化影响了蛋白质产物的氨基酸序列。发现cox3初级转录本的编辑改变了蛋白质-蛋白质相互作用位点的位置。这些结果再次证明了mRNA编辑在植物线粒体基因表达的转录后调控中的重要作用。