采用MPN-PCR法对发酵香肠中的单核细胞增生李斯特菌进行定量分析。
Quantification of Listeria monocytogenes in fermented sausages by MPN-PCR method.
作者信息
Martín B, Jofré A, Garriga M, Hugas M, Aymerich T
机构信息
Meat Technology Center, IRTA, Granja Camps i Armet, Girona, Spain.
出版信息
Lett Appl Microbiol. 2004;39(3):290-5. doi: 10.1111/j.1472-765X.2004.01580.x.
AIMS
To combine the principles of most-probable-number (MPN) statistics and the conventional PCR technique to enumerate Listeria monocytogenes in fermented sausages.
METHODS AND RESULTS
A simple method to enumerate L. monocytogenes in fermented sausages was developed and compared with direct plating in Palcam agar. Species-specific MPN-PCR, but not direct plating, made the enumeration of L. monocytogenes possible in all assayed samples.
CONCLUSIONS
MPN-PCR proved to be a rapid and reliable method for enumerating L. monocytogenes in fermented sausages, including low contaminated samples.
SIGNIFICANCE AND IMPACT OF THE STUDY
This MPN-PCR technique may facilitate the enumeration of L. monocytogenes for routine analyses in fermented sausages without excessive work.
目的
结合最大可能数（MPN）统计原理与传统聚合酶链反应（PCR）技术，对发酵香肠中的单核细胞增生李斯特菌进行计数。
方法与结果
开发了一种简单的方法来对发酵香肠中的单核细胞增生李斯特菌进行计数，并与在Palcam琼脂上直接平板计数法进行比较。种特异性MPN-PCR（而非直接平板计数法）使得在所有检测样品中对单核细胞增生李斯特菌进行计数成为可能。
结论
MPN-PCR被证明是一种快速且可靠的方法，可用于对发酵香肠中的单核细胞增生李斯特菌进行计数，包括低污染样品。
研究的意义与影响
这种MPN-PCR技术可能有助于在不过度增加工作量的情况下，对发酵香肠中的单核细胞增生李斯特菌进行常规分析计数。
Zotero 插件