作为仿生系统的化学微图案化自组装单分子层上DMPC脂质层的结构组织

Structural organization of DMPC lipid layers on chemically micropatterned self-assembled monolayers as biomimetic systems.

作者信息

Brechling A, Pohl M, Kleineberg U, Heinzmann U

机构信息

Faculty of Physics, University of Bielefeld, Universitaetsstr. 25, D-33501 Bielefeld, Germany.

出版信息

J Biotechnol. 2004 Aug 26;112(1-2):115-25. doi: 10.1016/j.jbiotec.2004.04.022.

DOI:10.1016/j.jbiotec.2004.04.022

PMID:15288947

Abstract

The growth structure of DMPC lipid layers on hydrophobic and hydrophilic alkylsilane-based self-assembled monolayers adsorbed on silicon has been investigated by means of X-ray reflectometry and atomic force microscopy. Hydrophilic modification of hydrophobically terminated ODS-SAMs has been achieved by dose-controlled irradiation with DUV light. While island formation of small DMPC bilayer islands is observed on hydrophobic SAM surfaces, closed layers of DMPC monolayers are formed on hydrophilic SAM surfaces. Furthermore, DMPC adsorption on chemically micropatterned substrates with alternating hydrophobic/hydrophilic surface properties has been studied by imaging ellipsometry and photoemission microscopy. Indication for at least partial bridging of hydrophobic areas by an adsorbed DMPC monolayer has been found.

摘要

通过X射线反射法和原子力显微镜研究了吸附在硅上的基于疏水和亲水烷基硅烷的自组装单分子层上DMPC脂质层的生长结构。通过用深紫外光进行剂量控制照射，实现了疏水端接的ODS-SAM的亲水化改性。在疏水SAM表面观察到小的DMPC双层岛的岛状形成，而在亲水SAM表面形成了DMPC单分子层的封闭层。此外，通过成像椭偏仪和光发射显微镜研究了DMPC在具有交替疏水/亲水表面性质的化学微图案化基底上的吸附。已发现吸附的DMPC单分子层至少部分桥接疏水区域的迹象。