感染性角膜炎的16S rDNA聚合酶链反应分析：病例系列
16S rDNA PCR analysis of infectious keratitis: a case series.
作者信息
Rudolph Thiemo, Welinder-Olsson Christina, Lind-Brandberg Lena, Stenevi Ulf
机构信息
Department of Ophthalmology, Sahlgrenska University Hospital, Gothenburg University, Gothenburg, Sweden.
出版信息
Acta Ophthalmol Scand. 2004 Aug;82(4):463-7. doi: 10.1111/j.1395-3907.2004.00303.x.
PURPOSE
To discuss the value of polymerase chain reaction (PCR) in the management of bacterial infectious keratitis.
METHODS
Corneal scrapings of four patients with severe infectious keratitis were analysed by culture and PCR of 16S ribosomal DNA (rDNA), followed by direct sequencing of the resulting amplicon. The medical history of the patients included laser-assisted in-situ keratomileusis (LASIK), penetrating keratoplasty (PKP) and trauma.
RESULTS
Using PCR we were able to identify a possible pathogen in all four cases, while bacterial cultures were either negative or did not correspond to the clinical picture. The identified bacteria were a Pseudomonas species, Abiotrophia defectiva, Stenotrophomonas maltophilia and Porphyromonas gingivalis.
CONCLUSIONS
Analysis of corneal scrapings by 16S rDNA PCR should be considered as a supplement to standard microbiological procedures. However, the results of this relatively new method have to be interpreted carefully.
目的
探讨聚合酶链反应（PCR）在细菌性感染性角膜炎治疗中的价值。
方法
对4例严重感染性角膜炎患者的角膜刮片进行培养及16S核糖体DNA（rDNA）的PCR分析，随后对扩增产物进行直接测序。患者的病史包括准分子激光原位角膜磨镶术（LASIK）、穿透性角膜移植术（PKP）和外伤。
结果
通过PCR我们能够在所有4例病例中鉴定出可能的病原体，而细菌培养结果要么为阴性，要么与临床表现不符。鉴定出的细菌分别为假单胞菌属、缺陷嗜氨基酸菌、嗜麦芽窄食单胞菌和牙龈卟啉单胞菌。
结论
16S rDNA PCR分析角膜刮片应被视为标准微生物学程序的补充。然而，这种相对较新的方法的结果必须谨慎解读。
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