Kerfeld C A, Wu Y P, Chan C, Krogmann D W, Yeates T O
UCLA-DOE Laboratory of Structural Biology and Molecular Medicine, 90095-1570, USA.
Acta Crystallogr D Biol Crystallogr. 1997 Nov 1;53(Pt 6):720-3. doi: 10.1107/S0907444997006999.
Crystals of a carotenoid protein from the cyanobacterium Arthrospira maxima have been grown in space group C2 with unit-cell dimensions a = 219.6, b = 40.3, c = 75.5 A and beta = 95.5 degrees. The crystals diffract X-rays to 2.3 A resolution and display unusual optical properties in polarized light that suggest that all of the carotenoid molecules in the crystals are oriented similarly. A slight increase in the concentration of a crystallization additive in the mother liquor induces macroscopic twinning, which is also visible when the crystals are illuminated with polarized light.
已在空间群C2中培养出极大节旋藻的类胡萝卜素蛋白晶体,其晶胞尺寸为a = 219.6、b = 40.3、c = 75.5 Å,β = 95.5°。这些晶体的X射线衍射分辨率达到2.3 Å,并且在偏振光下显示出异常的光学性质,这表明晶体中所有类胡萝卜素分子的取向相似。母液中结晶添加剂浓度的轻微增加会诱导宏观孪晶,当晶体用偏振光照射时也能看到这种孪晶。