来自打碗花的一种新型植物凝集素的结晶及初步X射线分析。
Crystallization and preliminary X-ray analysis of a novel plant lectin from Calystegia sepium.
作者信息
Wright L M, Wood S D, Reynolds C D, Rizkallah P J, Van Damme E J, Peumans W J
机构信息
School of Biomolecular Sciences, Liverpool John Moores University, England.
出版信息
Acta Crystallogr D Biol Crystallogr. 1997 Mar 1;53(Pt 2):220-1. doi: 10.1107/S0907444996010955.
A mannose-specific lectin from Calystegia sepium has been crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. The needle-shaped crystals are orthorhombic, space group C222(1) with cell dimensions a = 55.2(1), b = 55.9 (1), c = 196.1 (1) A. Fresh crystals diffract to 1.9 A resolution on a synchrotron radiation source. The asymmetric unit contains a dimer of two identical 16 kDa subunits with a packing density of 2.36 A(3) Da(-1). Intensity data have been observed beyond 2.0 A, but reasonable statistics restricted the usable range to 2.0 A.
采用悬滴气相扩散法，以硫酸铵作为沉淀剂，使来自打碗花（Calystegia sepium）的一种甘露糖特异性凝集素结晶。针状晶体为正交晶系，空间群为C222(1)，晶胞参数a = 55.2(1) Å，b = 55.9 (1) Å，c = 196.1 (1) Å。新鲜晶体在同步辐射源上的衍射分辨率可达1.9 Å。不对称单元包含由两个相同的16 kDa亚基组成的二聚体，堆积密度为2.36 ų Da⁻¹。已在2.0 Å以外观察到强度数据，但合理的统计将可用范围限制在2.0 Å。
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