幽门螺杆菌BabA2基因的克隆、表达及免疫原性
Cloning and expression and immunogenicity of Helicobacter pylori BabA2 gene.
作者信息
Bai Yang, Zhang Ya-Li, Chen Ye, Jin Jian-Feng, Zhang Zhao-Shan, Zhou Dian-Yuan
机构信息
PLA Institute for Digestive Medicine, Nanfang Hospital, the First Military Medical University, Guangzhou 510515, Guangdong Province, China.
出版信息
World J Gastroenterol. 2004 Sep 1;10(17):2560-2. doi: 10.3748/wjg.v10.i17.2560.
AIM
To construct a recombinant strain which expresses BabA of Helicobacter pylori (H pylori) and to study the immunogenicity of BabA.
METHODS
BabA2 DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E.coli strain. Furthermore, BabA immunogenicity was studied by animal test.
RESULTS
DNA sequence analysis showed the sequence of BabA2 DNA was the same as the one published by GenBank. The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA.
CONCLUSION
BabA recombinant protein may be an potential vaccine for control and treatment of H pylori infection.
目的
构建表达幽门螺杆菌（H pylori）BabA的重组菌株，并研究BabA的免疫原性。
方法
通过PCR扩增BabA2 DNA，并将其插入原核表达载体pET-22b(+)中，在BL21(DE3)大肠杆菌菌株中表达。此外，通过动物试验研究BabA的免疫原性。
结果
DNA序列分析表明，BabA2 DNA序列与GenBank公布的序列相同。BabA重组蛋白占细菌总蛋白的34.8%。幽门螺杆菌感染患者的血清以及用BabA自身免疫的Balb/c小鼠能够识别重组BabA（rBabA）。
结论
BabA重组蛋白可能是控制和治疗幽门螺杆菌感染的一种潜在疫苗。
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