大豆中一个NBS-LRR抗性基因同源物的鉴定

Characterization of an NBS-LRR resistance gene homologue from soybean.

作者信息

Wang Bangjun, Wang Yongjun, Wang Qiang, Luo Guangzuo, Zhang Zhigang, He Chaoying, He Si Jie, Zhang Jinsong, Gai Junyi, Chen Shouyi

机构信息

Plant Biotechnology Laboratory, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

出版信息

J Plant Physiol. 2004 Jul;161(7):815-22. doi: 10.1016/j.jplph.2004.01.007.

DOI:10.1016/j.jplph.2004.01.007

PMID:15310071

Abstract

Conserved motifs such as the nucleotide-binding site (NBS) were found in many characterized plant disease resistance genes. Based on the NBS domain, resistance gene analogs have been isolated in our previous study and were used as probes to screen a soybean (Glycine max) cDNA library. A full-length cDNA, KR4, was isolated by screening the library and rapid amplification of cDNA ends method. Sequence analysis revealed that the cDNA was 3818 bp in length and the open reading frame coded for a polypeptide of 1211 amino acids with an NBS and five leucine-rich repeats domains, which were identified by Pfam protein analysis. Sequence alignment showed that KR4 was similar to 12 protein of tomato. Southern analysis indicated that the KR4 gene had low copies in soybean genome and it was mapped on the molecular linkage group E. Its expression was also investigated and it was found that KR4 was induced by exogenous salicylic acid and responded upon infection of soybean mosaic virus strain N3.

摘要

在许多已鉴定的植物抗病基因中发现了诸如核苷酸结合位点（NBS）等保守基序。基于NBS结构域，我们在先前的研究中分离出了抗病基因类似物，并将其用作探针来筛选大豆（Glycine max）cDNA文库。通过筛选文库和cDNA末端快速扩增方法分离出了一个全长cDNA，即KR4。序列分析表明，该cDNA长度为3818 bp，开放阅读框编码一个由1211个氨基酸组成的多肽，具有一个NBS和五个富含亮氨酸重复序列结构域，这些结构域通过Pfam蛋白质分析得以鉴定。序列比对显示，KR4与番茄的12种蛋白质相似。Southern分析表明，KR4基因在大豆基因组中的拷贝数较低，并且它被定位在分子连锁群E上。还对其表达进行了研究，发现KR4受外源水杨酸诱导，并且在感染大豆花叶病毒株系N3时会产生响应。