Wan Xiao-yun, Shi Yi-fu, Li Juan-qing
Woman's Hospital, Zhejiang University, Hangzhou 310006, China.
Zhonghua Zhong Liu Za Zhi. 2004 May;26(5):275-8.
To evaluate the inhibitory effect of tumor suppressor PTEN on cell growth of endometrial carcinoma.
The exogenous wild PTEN cDNA via an adenoviral vector (Ad-PTEN) was introduced into Ishikawa cells. The expression of PTEN protein was detected by Western blot. The growth of Ishikawa cells was evaluated by trypan blue exclusion method and MTT.
The expression of PTEN protein was induced on day 1, and greatly increasing on day 3 - 5 after Ad-PTEN infection. The expression of PTEN significantly inhibited the growth of Ishikawa cells, and also significantly inhibited the growth of Ishikawa cells induced by IGF-II.
Adenovirus-mediated introduction of exogenous PTEN into human endometrial carcinoma cells can induce growth suppression. PTEN gene may be a novel therapeutic agent for endometrial carcinoma.
评估肿瘤抑制因子PTEN对子宫内膜癌细胞生长的抑制作用。
通过腺病毒载体（Ad-PTEN）将外源性野生型PTEN cDNA导入Ishikawa细胞。采用蛋白质免疫印迹法检测PTEN蛋白的表达。通过台盼蓝排斥法和MTT法评估Ishikawa细胞的生长情况。
Ad-PTEN感染后第1天诱导PTEN蛋白表达，第3至5天显著增加。PTEN的表达显著抑制Ishikawa细胞的生长，也显著抑制IGF-II诱导的Ishikawa细胞生长。
腺病毒介导的外源性PTEN导入人子宫内膜癌细胞可诱导生长抑制。PTEN基因可能是子宫内膜癌的一种新型治疗药物。
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