大鼠脊髓源性干细胞的体外培养与分化
[Cultivation and differentiation of spinal cord-derived stem cells in vitro in rats].
作者信息
Wang Dehua, Xu Jianguang, Jiang Hao
机构信息
Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai, 200032, PR China.
出版信息
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2004 Jul;18(4):247-9.
OBJECTIVE
To investigate the method of cultivation and the feature of differentiation of spinal cord-derived stem cells in vitro.
METHODS
The neural stem cells from spinal cord of 15 days fetal rats were harvested and cultivated in a serum-free limited medium. The stem cells were induced to differentiate and the results were identified by cellular immunohistochemistry.
RESULTS
Lots of stem cells were obtained from the spinal cord of fetal rats and the sphere of stem cells was formed about 10 days. Neural stem cells can give rise to mature neurons and astrocytes.
CONCLUSION
Epidermal growth factor/basic fibroblast growth factor serum-free limited medium can promote the proliferation activity of the stem cells. Spinal cord-derived stem cells can differentiate into glial cells and neurons.
目的
探讨脊髓源性干细胞的体外培养方法及分化特性。
方法
取15天胎鼠脊髓神经干细胞，在无血清限定培养基中培养。诱导干细胞分化，结果通过细胞免疫组织化学鉴定。
结果
从胎鼠脊髓获得大量干细胞，约10天形成干细胞球。神经干细胞可分化为成熟神经元和星形胶质细胞。
结论
表皮生长因子/碱性成纤维细胞生长因子无血清限定培养基可促进干细胞的增殖活性。脊髓源性干细胞可分化为神经胶质细胞和神经元。
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