Fisch P, Oettel K, Fudim N, Surfus J E, Malkovsky M, Sondel P M
Department of Human Oncology, University of Wisconsin, Madison 53792.
J Immunol. 1992 Apr 15;148(8):2315-23.
Human V gamma 9/V delta 2 T cells, the major subset of gamma/delta T cells in peripheral blood of adults, mediate proliferative and cytotoxic responses to Daudi Burkitt's lymphoma cells without previous in vitro exposure to Daudi. Our experiments show that some gamma/delta T cells coexpressing V gamma 9 and V delta 1 genes also react to Daudi cells in cytotoxic and proliferative assays. Expression of V gamma 9 is not sufficient for the recognition of Daudi cells because most gamma/delta T cells expressing V delta 1 paired with V gamma 9 or other V gamma genes neither kill Daudi cells nor proliferate to Daudi. V gamma 9/V delta 2 T cells do not proliferate to other cell lines such as K562 or Molt4 that are sensitive to MHC-unrestricted cytolysis by NK cells and by most IL-2-activated gamma/delta T cell clones. Cold target inhibition assays demonstrate that Daudi cells are stronger inhibitors than K562 and Molt4 of MHC-unrestricted lysis by V gamma 9/V delta 2 clones. However, cold Daudi cells are relatively weaker inhibitors of MHC-unrestricted lysis by NK cell clones, most gamma/delta T cell clones expressing V delta 1 and alpha/beta T cell clones. Thus, recognition by V gamma 9/V delta 2 T cells and certain V gamma 9/V delta 1 T cells of Daudi appears to involve a specific triggering pathway that is distinct from recognition by these gamma/delta T cells of Molt4, K562, and other target cells. NK cell clones and most other gamma/delta and alpha/beta T cell clones derived from the same normal volunteer blood donors do not show this specific interaction with Daudi cells. These data show that distinct subsets of human gamma/delta T cells recognize Daudi cells and support the idea that the gamma/delta TCR may be directly involved.
人类Vγ9/Vδ2 T细胞是成人外周血中γ/δ T细胞的主要亚群，在未事先体外接触Daudi细胞的情况下，介导对Daudi伯基特淋巴瘤细胞的增殖和细胞毒性反应。我们的实验表明，一些共表达Vγ9和Vδ1基因的γ/δ T细胞在细胞毒性和增殖试验中也对Daudi细胞产生反应。Vγ9的表达不足以识别Daudi细胞，因为大多数表达与Vγ9或其他Vγ基因配对的Vδ1的γ/δ T细胞既不杀伤Daudi细胞，也不对Daudi细胞产生增殖反应。Vγ9/Vδ2 T细胞不会对其他细胞系如K562或Molt4产生增殖反应，而NK细胞和大多数IL-2激活的γ/δ T细胞克隆对这些细胞系具有MHC非限制性细胞溶解作用。冷靶抑制试验表明，在Vγ9/Vδ2克隆介导的MHC非限制性裂解中，Daudi细胞比K562和Molt4是更强的抑制剂。然而，冷Daudi细胞对NK细胞克隆、大多数表达Vδ1的γ/δ T细胞克隆和α/β T细胞克隆介导的MHC非限制性裂解的抑制作用相对较弱。因此，Vγ9/Vδ2 T细胞和某些Vγ9/Vδ1 T细胞对Daudi细胞的识别似乎涉及一条特定的触发途径，该途径不同于这些γ/δ T细胞对Molt4、K562和其他靶细胞的识别。来自同一正常志愿者献血者的NK细胞克隆以及大多数其他γ/δ和α/β T细胞克隆与Daudi细胞没有这种特异性相互作用。这些数据表明，人类γ/δ T细胞的不同亚群识别Daudi细胞，并支持γ/δ TCR可能直接参与的观点。
