Gonçalves Eloisa da Graça do Rosario, Lopes Maria José Saraiva, de Oliveira Eurípedes Gomes, Hofer Ernesto
Departamento de Patologia, Universidade Federal do Maranhão, São Luis, MA.
Rev Soc Bras Med Trop. 2004 Jul-Aug;37(4):318-23. Epub 2004 Aug 20.
This study was carried from October 1997 to October 1998 with the purpose of investigating the link between the species Vibrio cholerae and zooplankton in the estuaries of rivers Anil and Bacanga in São Luis, Maranhão. Research of viable but non culturable forms of Vibrio cholerae O1, and analyze the correlation between pH values, salinity and water temperature with the presence of bacteria in zooplankton samples. The traditional method of cultivation and fluorescent antibody technique were applied to detect the bacteria. A total of 52 samples of zooplankton were collected. There was a predominance of Copepoda. The culture enabled 55 isolates of Vibrio cholerae non-O1. The serogroups O1 and O139 were identified in 37 (71.1%) and 17 (32.7%) samples respectively by the fluorescence. Viable but non culturable forms of Vibrio cholerae were detected in 70.8% of the samples studied. A significant correlation was established between salinity and the water's pH value and Vibrio cholerae.
本研究于1997年10月至1998年10月进行,目的是调查马兰热州圣路易斯市阿尼尔河和巴坎加河河口处霍乱弧菌与浮游动物之间的联系。研究霍乱弧菌O1的活的非可培养形式,并分析浮游动物样本中pH值、盐度和水温与细菌存在之间的相关性。采用传统培养方法和荧光抗体技术检测细菌。共采集了52份浮游动物样本。桡足类占优势。培养获得了55株非O1群霍乱弧菌分离株。通过荧光法分别在37份(71.1%)和17份(32.7%)样本中鉴定出O1群和O139群。在所研究的70.8%的样本中检测到了活的非可培养形式的霍乱弧菌。盐度、水体pH值与霍乱弧菌之间建立了显著相关性。