Hu Chunling, Qi Yipeng, Zhang Peng, Liu Xin, Xu Qin, Chen Xi
Key Laboratory of Virology, Ministry of Education, College of Life Science, Wuhan University, Wuhan 430072, Hubei Province, People's Republic of China.
Biochem Biophys Res Commun. 2004 Sep 24;322(3):794-802. doi: 10.1016/j.bbrc.2004.08.003.
Previous research showed that the expression of measles virus receptor CD46 was downregulated after expression of measles virus hemagglutinin protein on the surface of the virus infected cell or triggered by infected cell-to-cell contact. We reported here that the mRNA level of CD46 in MV infected cells was not changed which was tested by real-time quantitative PCR. To further analyse the surface expression alteration of CD46 after MV infection, flow cytometric analysis and indirect immunofluorescence were used to detect the protein level of CD46. Altogether, our results provided a demonstration that the expression of CD46 was not downregulated by the infection of MV strain S191 both on mRNA level and cellular surface protein level. Previous results reported that the "downregulation" of CD46 expression on the cell surface may take place because H protein masks the antibody recognition site on CD46 which results in "downregulation" of the expression of CD46.
先前的研究表明，在病毒感染细胞表面表达麻疹病毒血凝素蛋白后，或由感染的细胞间接触触发后，麻疹病毒受体CD46的表达会下调。我们在此报告，通过实时定量PCR检测发现，麻疹病毒感染细胞中CD46的mRNA水平没有变化。为了进一步分析麻疹病毒感染后CD46的表面表达变化，采用流式细胞术分析和间接免疫荧光法检测CD46的蛋白水平。总之，我们的结果表明，麻疹病毒S191株感染在mRNA水平和细胞表面蛋白水平上均未下调CD46的表达。先前的研究结果报道，细胞表面CD46表达的“下调”可能是因为H蛋白掩盖了CD46上的抗体识别位点，从而导致CD46表达的“下调”。
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