用于测定UDP-GlcpNAc:β-D-Galp-(1→4)-β-D-GlcpNAc（GlcNAc至Gal）β(1→3)-N-乙酰葡糖胺基转移酶活性的四糖受体的合成以及其酶促糖基化形成的五糖产物。

Synthesis of a tetrasaccharide acceptor for use in the assay of UDP-GlcpNAc:beta-D-Galp-(1----4)-beta-D-GlcpNAc (GlcNAc to Gal) beta(1----3)-N-acetylglucosaminyltransferase activity and the pentasaccharide product that would be formed by its enzymic glycosylation.

作者信息

Srivastava G, Hindsgaul O

机构信息

Department of Chemistry, University of Alberta, Edmonton, Canada.

出版信息

Carbohydr Res. 1992 Feb 7;224:83-93. doi: 10.1016/0008-6215(92)84095-a.

DOI:10.1016/0008-6215(92)84095-a

Abstract

The tetrasaccharide beta-D-Galp-(1----4)-beta-D-GlcpNAc-(1----6)-alpha-D- Manp-(1----6)-beta-D-Manp-OR (2) and the pentasaccharide beta-D-GlcpNAc-(1----3)-beta-D-Galp-(1----4)-beta-D- GlcpNAc-(1----6)-alpha-D-Manp-(1----6)-beta-D-Manp-OR (3), where R = (CH2)8COOMe, have been prepared by using combined chemical and enzymic procedures. Structure 2 is a substrate for UDP-GlcpNAc:beta-D-Galp-(1----4)- beta-D-GlcpNAc (GlcNAc to Gal) beta(1----3)-N-acetylglucosaminyltransferase, and 3 would be the product of its action. Antibodies raised against 3 are intended for use in an ELISA assay that would quantitate the enzymic conversion of immobilized 2 into 3.

摘要

四糖β-D-半乳糖基-（1→4）-β-D-氨基葡萄糖基-（1→6）-α-D-甘露糖基-（1→6）-β-D-甘露糖-OR（2）和五糖β-D-氨基葡萄糖基-（1→3）-β-D-半乳糖基-（1→4）-β-D-氨基葡萄糖基-（1→6）-α-D-甘露糖基-（1→6）-β-D-甘露糖-OR（3），其中R =（CH2）8COOMe，已通过化学和酶促联合方法制备。结构2是UDP-氨基葡萄糖：β-D-半乳糖基-（1→4）-β-D-氨基葡萄糖（氨基葡萄糖到半乳糖）β（1→3）-N-乙酰氨基葡萄糖转移酶的底物，3将是其作用产物。针对3产生的抗体用于ELISA测定，该测定将定量固定化的2酶促转化为3的过程。

相似文献

1

Synthesis of a tetrasaccharide acceptor for use in the assay of UDP-GlcpNAc:beta-D-Galp-(1----4)-beta-D-GlcpNAc (GlcNAc to Gal) beta(1----3)-N-acetylglucosaminyltransferase activity and the pentasaccharide product that would be formed by its enzymic glycosylation.

Carbohydr Res. 1992 Feb 7;224:83-93. doi: 10.1016/0008-6215(92)84095-a.

2

Combined chemical-enzymic synthesis of deoxygenated oligosaccharide analogs: transfer of deoxygenated D-GlcpNAc residues from their UDP-GlcpNAc derivatives using N-acetylglucosaminyltransferase I.

Carbohydr Res. 1990 Oct 25;207(2):259-76. doi: 10.1016/0008-6215(90)84053-w.

3

Elongation of both branches of biantennary backbones of oligo-(N-acetyllactosamino)glycans by human serum (1----3)-N-acetyl-beta-D- glucosaminyltransferase.

Carbohydr Res. 1992 Mar 16;226(1):155-74. doi: 10.1016/0008-6215(92)84061-v.

4

Acceptor-substrate recognition by N-acetylglucosaminyltransferase-V: critical role of the 4"-hydroxyl group in beta-D-GlcpNAc-(1-->2)-alpha-D-Manp(1-->6)-beta-D-Glcp-OR.

Carbohydr Res. 1993 Apr 23;243(1):139-64. doi: 10.1016/0008-6215(93)84087-m.

5

Synthesis of Hexp-(1-->4)-beta-D-GlcpNAc-(1-->2)-alpha-D-Manp-(1-->O) (CH2)7 CH3 probes for exploration of the substrate specificity of glycosyltransferases: Part II, Hex = 3-O-methyl-beta-D-Gal, 3-deoxy-beta-D-Gal, 3-deoxy-3-fluoro-beta-D-Gal, 3-amino-3-deoxy-beta-D-Gal, beta-D-Gul, alpha-L-Alt, or beta-L-Gal.

Carbohydr Res. 1997 Jan 17;297(3):209-27. doi: 10.1016/s0008-6215(96)00266-2.

6

Combined chemical-enzymic synthesis of a dideoxypentasaccharide for use in a study of the specificity of N-acetyl-glucosaminyltransferase-III.

Carbohydr Res. 1992 Mar 30;226(2):219-31. doi: 10.1016/0008-6215(92)84069-5.

7

Use of N-acetylglucosaminyltransferases I and II in the preparative synthesis of oligosaccharides.

Carbohydr Res. 1991 Mar 20;210:145-53. doi: 10.1016/0008-6215(91)80118-7.

8

UDP-N-trifluoroacetylglucosamine as an alternative substrate in N-acetylglucosaminyltransferase reactions.

Carbohydr Res. 1998 Jan;306(1-2):127-36. doi: 10.1016/s0008-6215(97)10033-7.

9

Synthesis of beta-D-GlcpNAc-(1-->2)-5a-carba-alpha-D-Man p-(1-->6)-beta-D- Glcp-O(CH2)7CH3: a reactive acceptor analog for N-acetylglucosaminyltransferase-V.

Carbohydr Res. 1995 Jul 11;271(2):197-205. doi: 10.1016/0008-6215(95)00083-6.

10

Enzymes involved in the biosynthesis of glycoconjugates. A UDP-2-acetamido-2-deoxy-D-glucose: beta-D-galactopyranosyl-(1 leads to 4)-saccharide (1 leads to 3)-2-acetamido-2-deoxy-beta-D- glucopyranosyltransferase in human serum.

Carbohydr Res. 1983 Aug 16;120:251-68. doi: 10.1016/0008-6215(83)88020-3.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。