[采用聚合酶链反应（PCR）并随后对16S核糖体RNA基因进行测序用于非结核分枝杆菌复合群的菌种鉴定]

[PCR with subsequent sequencing of the 16S gene rRNA in species identification of mycobacteria of the non-tuberculosis complex].

作者信息

Maĭorova A A, Stepanshina V N, Korobova O V, Shemiakin I G, Lazovskaia A L, Il'ina E A

出版信息

Mol Gen Mikrobiol Virusol. 2004(3):11-20.

Abstract

One hundred of mycobacterium cultures were assayed by the method of PCR with subsequent sequencing of the 16S rRNA region. The below mycobacterium species were identified: M. tuberculosis complex (n = 55), M. avium (n = 17), M. intracellulare (n = 4), M. scrofaleceum (n = 2), M. kansasii - M. gastri (n = 3), M. gordonae (n = 3), M. ulcerans - M. marinum (n = 1), M. smegmatis (m = 2), M. fortuitum (n = 11), M. peregrinum (n = 1) and M. chelonae - M. abscessus (n = 1). The method enabled the differentiation of species M. avium from M. intracellulare and M. peregrinum from M. fortuitum, which could not be differentiated by using the classic biochemical and bacteriological methods. Genetic heterogeneity of the mycobacterium strains of M. avium, M. fortuitum and M. gordonae was also established by PCR plus sequencing of the 16S rRNA region.

摘要

采用聚合酶链反应（PCR）方法对100份分枝杆菌培养物进行检测，并对16S rRNA区域进行后续测序。鉴定出以下分枝杆菌种类：结核分枝杆菌复合群（n = 55）、鸟分枝杆菌（n = 17）、胞内分枝杆菌（n = 4）、瘰疬分枝杆菌（n = 2）、堪萨斯分枝杆菌-胃分枝杆菌（n = 3）、戈登分枝杆菌（n = 3）、溃疡分枝杆菌-海分枝杆菌（n = 1）、耻垢分枝杆菌（m = 2）、偶然分枝杆菌（n = 11）、龟分枝杆菌（n = 1）和龟分枝杆菌-脓肿分枝杆菌（n = 1）。该方法能够区分鸟分枝杆菌与胞内分枝杆菌，以及龟分枝杆菌与偶然分枝杆菌，而使用经典的生化和细菌学方法无法进行区分。通过对16S rRNA区域进行PCR加测序，还确定了鸟分枝杆菌、偶然分枝杆菌和戈登分枝杆菌菌株的遗传异质性。