[川芎嗪对离体兔阴茎海绵体组织舒张作用的研究]

[Effect of chuanxiongzine on the relaxation of isolated rabbit corpus cavernosum tissues].

作者信息

Xiao Hengjun, Liu Jihong, Yin Chunping, Wang Tao, Chen Jun, Fan Longchang, Chen Zhi, Ye Zhangqun

机构信息

School of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

出版信息

Zhonghua Nan Ke Xue. 2004 Aug;10(8):636-9.

PMID:15362533

Abstract

OBJECTIVE

To investigate the effects of chuanxiongzine on the relaxation of isolated rabbit corpus cavernosum tissues.

METHODS

Observations were made on the relaxing effect of chuanxiongzine on phenylephrine (PE)-precontracted corpus cavernosum and cavernosal strips preincubated with L-NAME, and ODQ, using endothelium removal and experimental method of smooth muscle strips. The effect of chuanxiongzine on cAMP and cGMP levels in corpus cavernosum was measured by 125I radioimmunoassay.

RESULTS

Chuanxiongzine had concentration-dependent relaxing effect on the cavernosal muscle contraction induced by PE (EC50 1.58 x 10(-4) mol/L). This relaxing effect was partially antagonized by ODQ and not blocked by L-NAME or endothelium removal. In PE-precontracted cavernosal strips, chuanxiongzine induced relaxation accompanied with an increase in cAMP and cGMP levels.

CONCLUSION

Chuanxiongzine was effective in relaxing cavernosal muscle precontracted by PE in vitro. And its relaxing effect may be enhanced by increasing cGMP and cAMP levels in the corpus cavernosum.

摘要

目的

研究川芎嗪对离体兔阴茎海绵体组织舒张作用的影响。

方法

采用去内皮和平滑肌条实验方法，观察川芎嗪对苯肾上腺素（PE）预收缩的阴茎海绵体及预先用L-精氨酸甲酯（L-NAME）和1H-[1,2,4]恶唑并[4,3-a]喹嗪-1-one（ODQ）孵育的海绵体条带的舒张作用。用125I放射免疫分析法测定川芎嗪对阴茎海绵体中环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）水平的影响。