Peng Zheng, Li Rong, Li Li, Zhang Lei, Jin Ting
Department of General Surgery, General Hospital of PLA, Beijing 100853, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Sep;20(5):595-7.
AIM: To enhance antitumor activity of tumor-draining lymph node(TDLN) cells by autologous dendritic cells(DCs) in-vitro. METHODS: Peripheral blood mononuclear cells(PBMCs) were isolated from the patients with gastric adenocarcinoma and induced with GM-CSF, IL-4 and TNF-alpha to obtain mature DCs which were then sensitized by autologous tumor lysate. TDLN cells were isolated and purified from lymph nodes of the patients and incubated with IL-2. Then we designed three experiment groups: (1)first group: TDLN cells activated by sensitized DCs; (2) second group:TDLN cells activated by autologous tumor lysate; (3)third group: TDLN cells alone, which were used as control. Cytotoxic activity of TDLN cells to gastric adenocarcinoma cell line KATO3 and human melanoma cell line A375 were compared between 3 groups. RESULTS: The cytotoxicity of TDLN cells activated by sensitized DCs to KATO3 cells was significantly stronger than that of TDLN cells activated by autologous tumor lysate or TDLN cells alone. In contrast, for A375 cells, cytotoxic activities of three TDLN cells had no significant difference. CONCLUSION: Specific antitumor activity of TDLN cells can be enhanced significantly by activated autologous DCs.
目的：在体外通过自体树突状细胞（DCs）增强肿瘤引流淋巴结（TDLN）细胞的抗肿瘤活性。 方法：从胃腺癌患者中分离外周血单个核细胞（PBMCs），并用粒细胞-巨噬细胞集落刺激因子（GM-CSF）、白细胞介素-4（IL-4）和肿瘤坏死因子-α（TNF-α）诱导以获得成熟的DCs，然后用自体肿瘤裂解物使其致敏。从患者淋巴结中分离并纯化TDLN细胞，并用白细胞介素-2（IL-2）孵育。然后我们设计了三个实验组：（1）第一组：用致敏DCs激活的TDLN细胞；（2）第二组：用自体肿瘤裂解物激活的TDLN细胞；（3）第三组：单独的TDLN细胞，用作对照。比较三组TDLN细胞对胃腺癌细胞系KATO3和人黑色素瘤细胞系A375的细胞毒活性。 结果：致敏DCs激活的TDLN细胞对KATO3细胞的细胞毒性明显强于自体肿瘤裂解物激活的TDLN细胞或单独的TDLN细胞。相比之下，对于A375细胞，三种TDLN细胞的细胞毒活性没有显著差异。 结论：激活的自体DCs可显著增强TDLN细胞的特异性抗肿瘤活性。
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004-9
Nan Fang Yi Ke Da Xue Xue Bao. 2006-5
Zhonghua Zhong Liu Za Zhi. 2006-7
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