不稳定型心绞痛患者冠状动脉斑块中肾上腺髓质素免疫反应性及mRNA表达增加。

Increased adrenomedullin immunoreactivity and mRNA expression in coronary plaques obtained from patients with unstable angina.

作者信息

Ishikawa T, Hatakeyama K, Imamura T, Ito K, Hara S, Date H, Shibata Y, Hikichi Y, Asada Y, Eto T

机构信息

First Department of Internal Medicine, Miyazaki Medical College, University of Miyazaki, Miyazaki, Japan.

出版信息

Heart. 2004 Oct;90(10):1206-10. doi: 10.1136/hrt.2003.017921.

DOI:10.1136/hrt.2003.017921

PMID:15367525

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1768472/

Abstract

OBJECTIVE

To examine the expression and localisation of adrenomedullin in human coronary atherosclerotic lesions from patients with unstable angina (UAP) and stable angina (SAP), and to study the relation between adrenomedullin expression and plaque instability.

DESIGN

A retrospective observational study.

PATIENTS

Directional coronary atherectomy samples were obtained from 15 patients with UAP and 12 with SAP.

METHODS

The localisation of adrenomedullin was examined by immunohistochemistry, and adreno-medullin mRNA expression was measured by quantitative polymerase chain reaction.

RESULTS

Adrenomedullin immunoreactivity was preferentially localised in macrophages, intimal smooth muscle cells, and proliferated microvessels. The mean number of adrenomedullin positive cells in five high power fields (x 400) per specimen was higher in patients with UAP than in those with SAP (mean (SEM), 110 (13) v 76 (7); p < 0.05); and the ratio of adrenomedullin positive to total cells was higher in patients with UAP (43.0 (2.2)% v 34.2 (2.0)%; p < 0.01). More adrenomedullin mRNA was expressed in the plaque of patients with UAP than in those with SAP (60.4 (16.9)% v 9.7 (3.3)%; p < 0.01).

CONCLUSIONS

The findings suggest that adrenomedullin is involved in the development of atherosclerosis and plaque instability in human coronary arteries, in an autocrine or paracrine manner.

摘要

目的

检测肾上腺髓质素在不稳定型心绞痛（UAP）和稳定型心绞痛（SAP）患者的人类冠状动脉粥样硬化病变中的表达及定位，并研究肾上腺髓质素表达与斑块不稳定性之间的关系。

设计

一项回顾性观察研究。

患者

从15例UAP患者和12例SAP患者中获取定向冠状动脉斑块旋切术样本。

方法

采用免疫组织化学法检测肾上腺髓质素的定位，并用定量聚合酶链反应测量肾上腺髓质素mRNA的表达。

结果

肾上腺髓质素免疫反应性主要定位于巨噬细胞、内膜平滑肌细胞和增生的微血管。每个标本在五个高倍视野（×400）中肾上腺髓质素阳性细胞的平均数，UAP患者高于SAP患者（均值（标准误），110（13）比76（7）；p<0.05）；UAP患者肾上腺髓质素阳性细胞与总细胞的比例更高（43.0（2.2）%比34.2（2.0）%；p<0.01）。UAP患者斑块中表达的肾上腺髓质素mRNA比SAP患者更多（60.4（16.9）%比9.7（3.3）%；p<0.01）。