Tsav-Wua J A, Inyang C U, Akpapunam M A
College of Education, Katsina-Ala, Benue State, Makurdi, Nigeria.
Int J Food Sci Nutr. 2004 Jun;55(4):317-24. doi: 10.1080/09637480410001225238.
A study was conducted on the microbiological quality of traditionally fermented cassava flour 'kpor umilin' and a laboratory modification of the method developed with a view to upgrading the traditional process. Microbial analysis of samples from both processing methods was evaluated using standard microbiological methods. The bacterial count in the traditionally processed flours ranged from 2.7 x 10(3) to 1.2 x 10(7) cfu/g, while the modified sample had a count of 3.5 x 10(2) cfu/g. The predominant flora were Leuconostoc spp., Lactobacillus spp., Staphylococcus spp., Micrococcus spp., Bacillus spp., Streptococcus spp. and coliforms such as Escherichia coli. Other isolates were Enterobacter spp. and proteus spp. The sample from the modified method had a negligible (4 MPN/100 ml) coliform count. The yeast and mould count ranged from 1.9 x 10(3) cfu/g to 3.9 x 10(5) cfu/g in the traditionally processed samples as compared with 1.5 x 10(3) cfu/g in the sample from the modified process. Aspergillus spp., Penicillium spp., Saccharomyces spp. and Geotrichum candidum were the predominant fungi. The poor microbiological quality of 'kpor umilin', processed traditionally, calls for urgency in upgrading the processing and preservation methods as developed in this study as well as proper education of the local processors on good manufacturing practices.
对传统发酵木薯粉“kpor umilin”的微生物质量进行了一项研究,并对开发的方法进行了实验室改进,以期提升传统工艺。使用标准微生物方法对两种加工方法的样品进行微生物分析。传统加工面粉中的细菌计数范围为2.7×10³至1.2×10⁷ cfu/g,而改良样品的计数为3.5×10² cfu/g。主要菌群为明串珠菌属、乳杆菌属、葡萄球菌属、微球菌属、芽孢杆菌属、链球菌属以及大肠杆菌等大肠菌群。其他分离菌为肠杆菌属和变形杆菌属。改良方法的样品大肠菌群计数可忽略不计(4 MPN/100 ml)。传统加工样品中的酵母菌和霉菌计数范围为1.9×10³ cfu/g至3.9×10⁵ cfu/g,而改良工艺样品中的计数为1.5×10³ cfu/g。曲霉属、青霉属、酵母属和白地霉是主要真菌。传统加工的“kpor umilin”微生物质量较差,这就迫切需要按照本研究中开发的方法提升加工和保存方法,并对当地加工者进行良好生产规范方面的适当教育。