Galli C, Duchi R, Crotti G, Turini P, Ponderato N, Colleoni S, Lagutina I, Lazzari G
Laboratorio di Tecnologie della Riproduzione, CIZ srl, Istituto Sperimentale Italiano Lazzaro Spallanzani, Via Porcellasco 7/F, 26100 Cremona, Italy.
Vet Res Commun. 2004 Aug;28 Suppl 1:121-6. doi: 10.1023/b:verc.0000045389.44345.2e.
Many factors influence the efficiency of the in vitro embryo production technology in cattle but the most important are the physiological conditions of the donor and the culture protocols for oocyte maturation and fertilization and for embryo culture from zygote to blastocyst. Therefore, general factors such as age, body conditions and herd management play a pivotal role together with more specific factors such as reproductive soundness and ovarian cyclicity. Given that good quality and competent oocytes are available a complex series of processes, including oocyte maturation, fertilization and culture of the derived zygotes, must be completed to generate viable embryos.
许多因素会影响牛体外胚胎生产技术的效率,但最重要的是供体的生理状况以及卵母细胞成熟、受精和从合子到囊胚的胚胎培养的培养方案。因此,年龄、身体状况和畜群管理等一般因素与生殖健康和卵巢周期性等更具体的因素一起起着关键作用。鉴于有优质且合格的卵母细胞,必须完成一系列复杂的过程,包括卵母细胞成熟、受精以及对所得合子的培养,才能产生有活力的胚胎。