编码α-乳白蛋白的猪cDNA序列。

The sequence of a porcine cDNA encoding alpha-lactalbumin.

作者信息

Das Gupta N A, Alexander L J, Beattie C W

机构信息

Division of Surgical Oncology, University of Illinois, Chicago 60612.

出版信息

Gene. 1992 Jan 15;110(2):265-6. doi: 10.1016/0378-1119(92)90660-h.

DOI:10.1016/0378-1119(92)90660-h

PMID:1537566

Abstract

A cDNA clone encoding porcine alpha-lactalbumin (alpha LA) was isolated and sequenced. The longest clone was 688 nucleotides (nt) long and encoded a preprotein of 141 amino acids (aa) including a leader peptide of 19 aa. The porcine cDNA exhibited a nt similarity of between 72.2%-83.5% to other alpha LA cDNAs and an aa similarity of between 50.8%-85.2% with other alpha LA aa sequences. The derived aa sequence varied at three positions from a previously reported sequence for porcine alpha LA obtained by direct aa sequencing.

摘要

分离并测序了一个编码猪α-乳白蛋白（αLA）的cDNA克隆。最长的克隆长688个核苷酸（nt），编码一个141个氨基酸（aa）的前体蛋白，包括一个19个aa的前导肽。猪cDNA与其他αLA cDNA的核苷酸相似性在72.2%-83.5%之间，与其他αLA氨基酸序列的氨基酸相似性在50.8%-85.2%之间。推导的氨基酸序列与先前通过直接氨基酸测序获得的猪αLA序列在三个位置上有所不同。