发育中大鼠嗅球在出生后第20天TUNEL阳性细胞短暂增加。

Transient increase of TUNEL-positive cells on postnatal day 20 in the developing rat olfactory bulb.

作者信息

Saito Kyoko, Saito Shouichiro, Taniguchi Kazuyuki, Kobayashi Naoto, Terashita Takehiro, Shimokawa Tetsuya, Mominoki Katsumi, Miyawaki Kyojy, Chen Jie, Gao Shuang-Yan, Li Chun-Yu, Matsuda Seiji

机构信息

Division of Anatomy and Embryology, Department of Integrated Basic Medical Science, School of Medicine, Ehime University, Shitsukawa, Shigenobu-cho, Onsen-gun, Ehime 791-0295, Japan.

出版信息

Neurosci Res. 2004 Oct;50(2):219-25. doi: 10.1016/j.neures.2004.07.001.

DOI:10.1016/j.neures.2004.07.001

PMID:15380329

Abstract

In the developing central nervous system, apoptosis plays an important role in the normal organization of the neuronal circuit. The timing of neurogenesis, proliferation, and migration of the neurons in the developing olfactory bulb (OB) is well studied; however, the involvement of apoptosis in this process is not fully understood. In this study, we examined the changes in the distribution and the number of apoptotic cells in the rat OB during embryonic and postnatal periods, by using terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end-labeling (TUNEL) staining. Although the number of TUNEL-positive cells was relatively small during the embryonic period, it gradually increased after birth, and peaked on postnatal day 20 with statistical significance, especially in the granule cell layer of the main OB. This transient increase of TUNEL-positive cells on postnatal day 20 may be involved in a critical event during maturation of the OB.

摘要

在发育中的中枢神经系统中，细胞凋亡在神经回路的正常组织中起着重要作用。发育中的嗅球（OB）中神经元的神经发生、增殖和迁移时间已得到充分研究；然而，细胞凋亡在此过程中的参与情况尚未完全了解。在本研究中，我们通过使用末端脱氧核苷酸转移酶介导的dUTP-地高辛配基缺口末端标记（TUNEL）染色，检查了大鼠OB在胚胎期和出生后各时期凋亡细胞的分布和数量变化。虽然胚胎期TUNEL阳性细胞数量相对较少，但出生后逐渐增加，并在出生后第20天达到峰值，具有统计学意义，尤其是在主OB的颗粒细胞层。出生后第20天TUNEL阳性细胞的这种短暂增加可能与OB成熟过程中的一个关键事件有关。