[干扰素诱导基因淋巴细胞抗原6复合体E和四肽重复序列1与系统性红斑狼疮患者的临床特征相关]

[Interferon-inducible genes lymphocyte antigen 6 complex E and tetratricopeptide repeats 1 are correlated with clinical features of patients with systemic lupus erythematosus].

作者信息

Tang Jian-ping, Gu Yue-ying, Shen Nan, Ye Shuang, Qian Jie, Hua Jing, Chen Xing-guo, Chen Shun-le, Bao Chun-de, Wang Yuan, Zhang Wei

机构信息

Shanghai Clinical Centre of Rheumatic Diseases and Institute of Rheumatology, Department of Rheumatology, Renji Hospital, Shanghai Second Medical University, Shanghai 200001, China.

出版信息

Zhonghua Yi Xue Za Zhi. 2004 Jul 17;84(14):1157-60.

PMID:15387975

Abstract

OBJECTIVE

To investigate the expression levels of lymphocyte antigen 6 complex, locus E (LY6E) and interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) genes in the peripheral blood mononuclear cells (PBMCs) of patients with systemic lupus erythematosus (SLE), and to evaluate the relations between these gene expression levels and disease activity.

METHODS

The clinical data of 144 SLE patients, 27 non-SLE patients with rheumatic diseases, and 59 normal controls were collected. The SLE patients were further divided into 2 subgroups: active SLE group (n = 87) and non-active SLE group (n = 57) according to SLEDAI scores. Specimens of peripheral blood were drawn; total RNA was extracted and transcribed into cDNA. Sybr green dye based real-time quantitative PCR method was used to compare the expression levels (indicated as-DeltaDeltaCT value) of LY6E and IFIT1 in patients with SLE and those in the controls.

RESULTS

(1) The-DeltaDeltaCT value of LY6E expression level of the SLE patients was 5.4760 +/- 1.9806, significantly higher than those of the non-SLE patients (3.4323 +/- 1.7456) and normal controls (4.5198 +/- 1.6359, both P = 0.001). (2) The-DeltaDeltaCT value of LY6E and IFIT1 mRNA expression of the active SLE patients were 6.1960 +/- 1.7729 and 6.4997 +/- 2.6297 respectively, significantly higher than those observed in the inactive SLE patients (4.3770 +/- 1.7764 and 4.1327 +/- 2.6044 respectively, both P = 0.000). The-DeltaDeltaCT values of LY6E and IFIT1 mRNA of the SLE patients were correlated with the SLEDAI scores, and with the numbers of matched criteria used in the diagnosis of SLE (P < 0.001).

CONCLUSION

As IFN-induced genes, elevated expression of LY6E mRNA is specific to diagnosis of SLE. The real time expression levels of LY6E and IFIT1 genes are associated with SLE disease activity. To inhibit the expression of LY6E and IFIT1 may become a novel therapeutic approach for SLE.

摘要

目的

研究系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMCs）中淋巴细胞抗原6复合体E位点（LY6E）和含四肽重复序列的干扰素诱导蛋白1（IFIT1）基因的表达水平，并评估这些基因表达水平与疾病活动度之间的关系。

方法

收集144例SLE患者、27例非SLE风湿性疾病患者及59例正常对照者的临床资料。根据SLE疾病活动指数（SLEDAI）评分将SLE患者进一步分为2个亚组：活动期SLE组（n = 87）和非活动期SLE组（n = 57）。采集外周血标本；提取总RNA并转录为cDNA。采用基于Sybr green染料的实时定量PCR方法比较SLE患者与对照者中LY6E和IFIT1的表达水平（以-ΔΔCT值表示）。

结果

（1）SLE患者LY6E表达水平的-ΔΔCT值为5.4760±1.9806，显著高于非SLE患者（3.4323±1.7456）和正常对照者（4.5198±1.6359，P均=0.001）。（2）活动期SLE患者LY6E和IFIT1 mRNA表达的-ΔΔCT值分别为6.1960±1.7729和6.4997±2.6297，显著高于非活动期SLE患者（分别为4.3770±1.7764和4.1327±2.6044，P均=0.000）。SLE患者LY6E和IFIT1 mRNA的-ΔΔCT值与SLEDAI评分以及SLE诊断中符合标准的数量相关（P<0.001）。