人类巨噬细胞集落刺激因子基因重新定位于染色体1p13 - 21。

Reassignment of the human macrophage colony stimulating factor gene to chromosome 1p13-21.

作者信息

Saltman D L, Dolganov G M, Hinton L M, Lovett M

机构信息

Department of Molecular Genetics, Genelabs Incorporated, Redwood City, CA 94063.

出版信息

Biochem Biophys Res Commun. 1992 Feb 14;182(3):1139-43. doi: 10.1016/0006-291x(92)91850-p.

DOI:10.1016/0006-291x(92)91850-p

PMID:1540160

Abstract

Macrophage colony stimulating factor (CSF-1) is a member of a family of glycoproteins that are necessary for the normal proliferation and differentiation of myeloid progenitor cells. The human CSF-1 gene has previously been assigned to chromosome 5 using somatic cell hybrids, and further localized to 5q33 by in situ hybridization with a 3H labelled cDNA probe. However, the murine macrophage colony stimulating factor gene (csfm) has been localized to a region on mouse chromosome 3 which was previously shown to be syntenic with the proximal region of 1p and not 5q. Using a human genomic DNA clone that contains the CSF-1 gene, we have localized CSF-1 to chromosome 1p13-21 by fluorescence in situ hybridization. The reassignment of the CSF-1 gene argues against its involvement in myeloid disorders with deletions of the long arm of chromosome 5.

摘要

巨噬细胞集落刺激因子（CSF-1）是一类糖蛋白家族的成员，这些糖蛋白对于髓系祖细胞的正常增殖和分化是必需的。先前利用体细胞杂种已将人CSF-1基因定位于5号染色体，并且通过用3H标记的cDNA探针进行原位杂交进一步定位于5q33。然而，小鼠巨噬细胞集落刺激因子基因（csfm）已定位于小鼠3号染色体上的一个区域，该区域先前显示与1p近端区域而非5q是同线的。利用一个包含CSF-1基因的人类基因组DNA克隆，我们通过荧光原位杂交将CSF-1定位于1p13 - 21染色体。CSF-1基因的重新定位表明它与5号染色体长臂缺失的髓系疾病无关。