发育过程中鸡视网膜色素上皮中乙酰胆碱酯酶和丁酰胆碱酯酶活性的表征
Characterization of acetylcholinesterase and butyrylcholinesterase activities in retinal chick pigment epithelium during development.
作者信息
Salceda R, Martinez M T
机构信息
Departamento de Neurociencias, Universidad Nacional Autónoma de México, D.F.
出版信息
Exp Eye Res. 1992 Jan;54(1):17-22. doi: 10.1016/0014-4835(92)90064-y.
The presence of acetylcholinesterase and butyrylcholinesterase was studied in chick retinal pigment epithelium. Acetylcholinesterase activity was 13 times higher than that of butyrylcholinesterase. The former showed a Km of 290 microM and a vmax of 45.4 nmol mg-1 protein min-1, while the latter showed two apparent Km values (132 microM and 666 microM). Studies on subcellular distribution revealed that both enzymes are associated with membranes. During the embryonic development butyrylcholinesterase activity decreased, while acetylcholinesterase activity increased. The possibility that these changes are related to the proliferation and differentiation processes is discussed.
研究了鸡视网膜色素上皮中乙酰胆碱酯酶和丁酰胆碱酯酶的存在情况。乙酰胆碱酯酶活性比丁酰胆碱酯酶高13倍。前者的Km为290微摩尔，vmax为45.4纳摩尔/毫克蛋白质/分钟，而后者显示出两个明显的Km值（132微摩尔和666微摩尔）。亚细胞分布研究表明，这两种酶都与膜相关。在胚胎发育过程中，丁酰胆碱酯酶活性降低，而乙酰胆碱酯酶活性增加。讨论了这些变化与增殖和分化过程相关的可能性。
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