来自日本对虾（Marsupenaeus japonicus）的丝氨酸蛋白酶同源物的肽聚糖诱导表达。
Peptidoglycan inducible expression of a serine proteinase homologue from kuruma shrimp (Marsupenaeus japonicus).
作者信息
Rattanachai Achara, Hirono Ikuo, Ohira Tsuyoshi, Takahashi Yukinori, Aoki Takashi
机构信息
Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
出版信息
Fish Shellfish Immunol. 2005 Jan;18(1):39-48. doi: 10.1016/j.fsi.2004.05.002.
A cDNA encoding a serine proteinase homologue of kuruma shrimp (Marsupenaeus japonicus) was cloned. The 1257 bp cDNA encodes a 339 amino acid putative peptide, with a signal sequence of 16 amino acid residues. The deduced amino acid sequence is 42-67% similar to the immune-related serine proteinases and serine proteinase homologues of arthropods. It contains catalytic triad residues in the putative catalytic domain except for one substitution of Ser by a Gly residue. The six cysteine residues that form three disulphide bridges in most serine proteinases were conserved. The M. japonicus serine proteinase homologue was mainly expressed in haemocytes, in which expression dramatically increased after 3 days feeding with peptidoglycan at 0.2 mg kg(-1) shrimp body weight per day.
克隆了一个编码日本对虾丝氨酸蛋白酶同源物的cDNA。该1257 bp的cDNA编码一个含339个氨基酸的假定肽，带有一个16个氨基酸残基的信号序列。推导的氨基酸序列与节肢动物免疫相关丝氨酸蛋白酶和丝氨酸蛋白酶同源物有42%-67%的相似性。在假定的催化结构域中，除了一个丝氨酸残基被甘氨酸残基取代外，它还含有催化三联体残基。在大多数丝氨酸蛋白酶中形成三个二硫键的六个半胱氨酸残基是保守的。日本对虾丝氨酸蛋白酶同源物主要在血细胞中表达，在用肽聚糖以每天每千克虾体重0.2 mg的剂量投喂3天后，其表达显著增加。
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