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[作为生物纳米技术研究对象的细菌和古细菌表面层]

[Bacterial and archaeal S-layers as object of bionanotechnology].

作者信息

Debabov V G

出版信息

Mol Biol (Mosk). 2004 Jul-Aug;38(4):578-91.

Abstract

Many species of Bacteria and Archaea posses a regularly structured surface layers (S-layers) as outermost cell envelope component. S-layers composed of a single protein or glycoprotein species. The individual subunits of S-layers interact with each other and with the supporting bacterial envelope component through non-covalent forces. Pores in the crystalline protein network are with mean diameter of 2-6 nm, the thickness of S-layer is 5-10 nm. The isolated S-layer subunits reassemble into two-dimensional crystalline arrays in solution, on solid supports, on planar lipid films. These unique features of S-layers have led to a broad spectrum applications. This review focuses on the structural properties S-layers and S-proteins and their applications with accent to using this structures in nanobiotechnology.

摘要

许多细菌和古菌物种都拥有规则结构的表面层(S层)作为最外层的细胞包膜成分。S层由单一蛋白质或糖蛋白组成。S层的各个亚基通过非共价力相互作用,并与支持性的细菌包膜成分相互作用。晶体蛋白网络中的孔平均直径为2-6纳米,S层厚度为5-10纳米。分离的S层亚基在溶液中、固体支持物上、平面脂质膜上重新组装成二维晶体阵列。S层的这些独特特性已导致了广泛的应用。本综述重点关注S层和S蛋白的结构特性及其应用,尤其强调在纳米生物技术中使用这些结构。

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