Suppr超能文献

[哈萨克斯坦不同地区的三个哈萨克族人群以及哈萨克斯坦阿尔泰古代人群DNA样本中线粒体基因组非编码区的多态性]

[Polymorphism of mitochondrial genome noncoding regions in the three Kazakh populations inhabited different areas of Kazakhstan and in the samples of DNA from ancient people of Kazakhstan Altai].

作者信息

Aĭtkhozhina N A, Dzisiuk N V, Liudvikova E K

出版信息

Mol Biol (Mosk). 2004 Jul-Aug;38(4):592-601.

Abstract

Polymorphism of major noncoding region of mitochondrial DNA (mtDNA D-loop, 528 bp in length) from the three modem kazakh populations and from DNA samples of ancient people inhabited modern Kazakhstani Altai were studied. PCR and RFLP analysis of 13 sites of restriction--BamHI, EcoRV, Sau3AI (1 restriction site), KpnI (2 sites), HaeIII (3 sites), RsaI (5 restriction sites), were carried out. The distribution of each site frequencies was determined. Nucleotide diversity (h) and genetic distance between different kazakh population and other populations of world were estimated. The same RFLP analysis of the mitochondrial DNA control region was carried out for the paleogenomic samples. It was shown that two samples of ancient mitochondrial DNA were monomorphous throughout all analyzed restriction sites.

摘要

对三个现代哈萨克人群体以及居住在现代哈萨克斯坦阿尔泰山地区古代居民的DNA样本中的线粒体DNA主要非编码区(mtDNA D环,长度为528bp)的多态性进行了研究。对13个限制性位点——BamHI、EcoRV、Sau3AI(1个限制性位点)、KpnI(2个位点)、HaeIII(3个位点)、RsaI(5个限制性位点)进行了PCR和RFLP分析。确定了每个位点频率的分布。估计了不同哈萨克人群体与世界其他群体之间的核苷酸多样性(h)和遗传距离。对古基因组样本进行了相同的线粒体DNA控制区RFLP分析。结果表明,两个古代线粒体DNA样本在所有分析的限制性位点上都是单态的。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验