Aĭtkhozhina N A, Dzisiuk N V, Liudvikova E K
Mol Biol (Mosk). 2004 Jul-Aug;38(4):592-601.
Polymorphism of major noncoding region of mitochondrial DNA (mtDNA D-loop, 528 bp in length) from the three modem kazakh populations and from DNA samples of ancient people inhabited modern Kazakhstani Altai were studied. PCR and RFLP analysis of 13 sites of restriction--BamHI, EcoRV, Sau3AI (1 restriction site), KpnI (2 sites), HaeIII (3 sites), RsaI (5 restriction sites), were carried out. The distribution of each site frequencies was determined. Nucleotide diversity (h) and genetic distance between different kazakh population and other populations of world were estimated. The same RFLP analysis of the mitochondrial DNA control region was carried out for the paleogenomic samples. It was shown that two samples of ancient mitochondrial DNA were monomorphous throughout all analyzed restriction sites.
对三个现代哈萨克人群体以及居住在现代哈萨克斯坦阿尔泰山地区古代居民的DNA样本中的线粒体DNA主要非编码区(mtDNA D环,长度为528bp)的多态性进行了研究。对13个限制性位点——BamHI、EcoRV、Sau3AI(1个限制性位点)、KpnI(2个位点)、HaeIII(3个位点)、RsaI(5个限制性位点)进行了PCR和RFLP分析。确定了每个位点频率的分布。估计了不同哈萨克人群体与世界其他群体之间的核苷酸多样性(h)和遗传距离。对古基因组样本进行了相同的线粒体DNA控制区RFLP分析。结果表明,两个古代线粒体DNA样本在所有分析的限制性位点上都是单态的。