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[大豆根瘤菌土著种群对引入菌株日本慢生根瘤菌634b共生活性的影响]

[Effect of aboriginal populations of soy nodule bacteria on symbiotic activity of introduced strain Bradyrhizobium japonicum 634b].

作者信息

Patyka V P, Krutylo D V, Kovalevs'ka T M

出版信息

Mikrobiol Z. 2004 May-Jun;66(3):14-21.

Abstract

The method of limit dilutions was used to determine the number of soy nodule bacteria in soils of different regions of Ukraine. Symbiotic properties of aboriginal populations of soy rhizobia have been studied under the conditions of vegetation researches, their effect on activity of the introduced strain B. japonicum 634b has been shown. Numerous aboriginal populations of soy nodule bacteria (200-3500 units per 1 g of soil) have been found in soils of fields where soy was sown during a long period of time. It is shown that they provide for the highest degree of plants infection. These populations can compete with the standard strain. Activity of formation of symbiotic apparatus in the variants with inoculation of B. japonicum 634b remains invariable or something decreases. It has been established that populations of nodule bacteria are not numerous (20 units per 1 g of soil) or absolutely absent in the field soils where soy growing is only started. Seeds inoculation by the productive strain under conditions of these soils favours considerable increase of the number of nodules (1.3-22.0 times) and their nitrogenase activity (1.2-1.9 times).

摘要

采用有限稀释法测定了乌克兰不同地区土壤中的大豆根瘤菌数量。在植被研究条件下,对大豆根瘤菌土著群体的共生特性进行了研究,结果表明它们对引入菌株日本慢生根瘤菌634b的活性有影响。在长期种植大豆的田间土壤中发现了大量的大豆根瘤菌土著群体(每克土壤200 - 3500个单位)。结果表明,它们能使植物获得最高程度的感染。这些群体能够与标准菌株竞争。接种日本慢生根瘤菌634b的变体中,共生器官形成的活性保持不变或有所下降。已经确定,在刚开始种植大豆的田间土壤中,根瘤菌群体数量不多(每克土壤20个单位)或完全没有。在这些土壤条件下,用生产菌株接种种子有利于根瘤数量显著增加(1.3 - 22.0倍)及其固氮酶活性提高(1.2 - 1.9倍)。

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