使用原子力显微镜监测分子信标/DNA相互作用。

Monitoring molecular beacon/DNA interactions using atomic force microscopy.

作者信息

Jin Yan, Wang Kemin, Tan Weihong, Wu Ping, Wang Qing, Huang Hongmei, Huang Shasheng, Tang Zhiwen, Guo Qiuping

机构信息

Biomedical Engineering Center, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.

出版信息

Anal Chem. 2004 Oct 1;76(19):5721-5. doi: 10.1021/ac049933u.

DOI:10.1021/ac049933u

PMID:15456291

Abstract

The molecular beacon (MB) is a new fluorescence probe containing a single-stranded oligonucleotide with a probe sequence embedded in complementary sequences that form a hairpin stem. Due to the inherent fluorescent signal transduction mechanism, an MB functions as a sensitive probe with a high signal-to-background ratio for real-time monitoring and provides a variety of exciting opportunities in DNA, RNA, and protein studies. To better understand the properties of MBs, the specific interactions between MB and target DNA (complementary and one-base mismatch) have been directly investigated by atomic force microscopy. The interaction force between a linear DNA probe and the target DNA was also detected and compared to that between MB and target DNA. The results demonstrate the high specificity of the MB/target DNA compared to the linear DNA/target DNA interaction.

摘要

分子信标（MB）是一种新型荧光探针，它包含一个单链寡核苷酸，其探针序列嵌入形成发夹茎的互补序列中。由于其固有的荧光信号转导机制，分子信标作为一种灵敏的探针，具有高信噪比，可用于实时监测，并在DNA、RNA和蛋白质研究中提供了各种令人兴奋的机会。为了更好地了解分子信标的特性，通过原子力显微镜直接研究了分子信标与靶DNA（互补和单碱基错配）之间的特异性相互作用。还检测了线性DNA探针与靶DNA之间的相互作用力，并与分子信标和靶DNA之间的相互作用力进行了比较。结果表明，与线性DNA/靶DNA相互作用相比，分子信标/靶DNA具有更高的特异性。