吗啡依赖和戒断对大鼠脑内神经甾体水平的影响。

Effects of morphine dependence and withdrawal on levels of neurosteroids in rat brain.

作者信息

Yan Cai-Zhen, Hou Yan-Ning

机构信息

Bathune International Peace Hospital, Hebei Medical University,Shijiazhuang 050082, China.

出版信息

Acta Pharmacol Sin. 2004 Oct;25(10):1285-91.

PMID:15456529

Abstract

AIM

To investigate the effects of morphine dependence and withdrawal on the concentrations of neurosteroids in rat brain.

METHODS

A method of simultaneous quantification of neurosteroids by gas chromatography-mass spectrometry (GC-MS) had been established.

RESULTS

The chronic morphine administration (ip) resulted in a marked decrease in the brain concentrations of pregnenolone (PREG), progesterone (PROG), and pregenenolone sulfate (PREGS) in rats killed 6 h after the last treatment. In contrast, there were no significant effects of morphine dependence on the brain concentrations of allopregnanolone (AP), dihydroepiandrosterone (DHEA), and dihydroepiandrosterone sulfate (DHEAS). Naloxone-induced withdrawal produced a significant increase in the concentrations of PREG, PROG, AP, DHEA, PREGS, and DHEAS as compared with the control group.

CONCLUSION

Morphine dependence and withdrawal affected the concentrations of neurosteroids in rat brain, which suggests that endogenous neurosteroids in brain might be related to the development of morphine dependence and withdrawal.

摘要

目的

研究吗啡依赖和戒断对大鼠脑内神经甾体浓度的影响。

方法

建立了气相色谱 - 质谱联用（GC-MS）同时定量神经甾体的方法。

结果

末次给药6小时后处死的大鼠，慢性腹腔注射吗啡导致孕烯醇酮（PREG）、孕酮（PROG）和硫酸孕烯醇酮（PREGS）的脑内浓度显著降低。相比之下，吗啡依赖对别孕烷醇酮（AP）、脱氢表雄酮（DHEA）和硫酸脱氢表雄酮（DHEAS）的脑内浓度无显著影响。与对照组相比，纳洛酮诱发的戒断使PREG、PROG、AP、DHEA、PREGS和DHEAS的浓度显著升高。