通过原位植入组织学完整的组织建立人肝细胞癌裸鼠模型。
Nude mice model of human hepatocellular carcinoma via orthotopic implantation of histologically intact tissue.
作者信息
Gao Yong-Shun, Chen Xiao-Ping, Li Kai-Yan, Wu Zai-De
机构信息
Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
出版信息
World J Gastroenterol. 2004 Nov 1;10(21):3107-11. doi: 10.3748/wjg.v10.i21.3107.
AIM
To establish a nude mice model of human hepatocellular carcinoma (HCC) via orthotopic implantation of histologically intact tissue, in order to study biologic features of HCC in vivo and to direct clinical treatment respectively.
METHODS
Histologically intact fresh specimens of HCC were orthotopically implanted in nude mice (BALB/c, nu/nu). Survival rate and growth curve were investigated with B-ultrasound. Morphological characteristics of pathology and spontaneous metastatic rates were detected with microscopy. Expression of multidrug resistance genes studied with immunohistochemical method and RT-PCR, and other biologic features of implanted tumor were observed and compared with human HCC specimens.
RESULTS
Out of the specimens from two patients with HCC, only one specimen survived in nude mice. The orthotopic implantation tumor survival rate, spontaneous intrahepatic metastatic rate, pulmonary metastatic rate and bone metastases rate were 100%, 75.0%, 37.5% and 37.5% respectively in the first passage. AFP was kept on secreting and increasing with the size of the tumor. The morphological characteristics and biologic features were similar to the donor's, the protein and mRNA of MDR1 and LRP were expressed in tumors of the model and the donor, and there was no significant difference between them (P>0.05).
CONCLUSION
The model of nude mice with orthotopic implantation of histologically intact HCC tissue is an ideal model to study biologic features of HCC in vivo and to direct clinical treatment.
目的
通过原位植入组织学完整的组织建立人肝细胞癌（HCC）裸鼠模型，以分别研究HCC的体内生物学特性并指导临床治疗。
方法
将组织学完整的新鲜HCC标本原位植入裸鼠（BALB/c，nu/nu）。用B超观察生存率和生长曲线。用显微镜检测病理形态特征和自发转移率。用免疫组化法和RT-PCR研究多药耐药基因的表达，并观察植入肿瘤的其他生物学特性，并与人类HCC标本进行比较。
结果
在两名HCC患者的标本中，只有一个标本在裸鼠中存活。第一代原位植入肿瘤的生存率、肝内自发转移率、肺转移率和骨转移率分别为100%、75.0%、37.5%和37.5%。甲胎蛋白随肿瘤大小持续分泌并增加。形态特征和生物学特性与供体相似，模型和供体肿瘤中均表达MDR1和LRP的蛋白质和mRNA，两者之间无显著差异（P>0.05）。
结论
组织学完整的HCC组织原位植入裸鼠模型是研究HCC体内生物学特性并指导临床治疗的理想模型。
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