Liu Gongping, Shi Nian, Ma Ronghong
Tongji Medical College of Huazhong Science and Technology University, Wuhan 430030, China.
Wei Sheng Yan Jiu. 2004 Jul;33(4):390-3.
To study the effects of pyrethroids on tyrosine hydroxylase in nigrostriatum of male rats.
Different doses of permethrin and deltamethrin in corn oil were administered to rats by gavage for ten days, then PLC-Fluorescence detection was used to analyzed the contents of dopamine (DA), DOPAC and HVA in the Substantial Nigra and Striatum. The activities of TH were also detected by HPLC-Fluorescence detection. TH mRNA or TH protein levels were measured by RT-PCR and immunohistochemistry method.
Contents of DA in Striatum in 1/10LD50 DM group have a significant change, the contents of DOPAC and the turnover rate of DA all increased (P < 0.05), but DA, DOPAC and (DOPAC + HVA)/DA did not have any significant change in Substantial Nigra. The activities and mRNA level of TH had obvious decrease in two DM groups, the TH protein levels also significantly decreased in 1/10LD50 DM group.
Deltamethrin could decrease the activities, mRNA and protein levels in nigraostriatum of rats.
研究拟除虫菊酯对雄性大鼠黑质纹状体中酪氨酸羟化酶的影响。
将不同剂量的氯菊酯和溴氰菊酯溶于玉米油中，经口灌胃给予大鼠，连续10天，然后采用PLC-荧光检测法分析黑质和纹状体中多巴胺（DA）、3,4-二羟基苯乙酸（DOPAC）和高香草酸（HVA）的含量。同时采用HPLC-荧光检测法检测酪氨酸羟化酶（TH）的活性。通过逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法测定TH mRNA或TH蛋白水平。
1/10LD50溴氰菊酯组纹状体中DA含量有显著变化，DOPAC含量及DA周转率均升高（P<0.05），但黑质中DA、DOPAC及（DOPAC+HVA）/DA无明显变化。两个溴氰菊酯组TH的活性和mRNA水平明显降低，1/10LD50溴氰菊酯组TH蛋白水平也显著降低。
溴氰菊酯可降低大鼠黑质纹状体中TH的活性、mRNA及蛋白水平。
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