鸡贫血病毒在MSB-1细胞中进行细胞培养传代后的遗传多样性。

Genetic diversity of chicken anemia virus following cell culture passaging in MSB-1 cells.

作者信息

Hasmah M S, Omar A R, Wan K F, Hair-Bejo M, Aini I

机构信息

Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

出版信息

Acta Virol. 2004;48(2):85-9.

PMID:15462283

Abstract

It has been shown that a chicken anemia virus (CAV) isolates which had undergone 60 passages in MSB-1 cells (SMSC-1/P60, 3-1/P60) acquired 33-66 nucleotide substitutions at the coding region resulting in 13-16 amino acid changes as compared to the CAV isolates passaged only 5 times in MSB-1 cells (SMSC-1 and 3-1) (Chowdhury et al., Arch. Virol. 148, 2437-2448, 2003). In this study we found that a low CAV (BL-5) and a high CAV passage (BL-5/P90) differed by only 15 nucleotide substitutions resulting in 11 amino acid changes. Phylogenetic analysis based on VP1 also revealed that both isolates were close to each other but not to other CAV isolates from Malaysia, namely SMSC-1 and 3-1.

摘要

研究表明，鸡贫血病毒（CAV）分离株在MSB - 1细胞中传代60次（SMSC - 1/P60、3 - 1/P60）后，与仅在MSB - 1细胞中传代5次的CAV分离株（SMSC - 1和3 - 1）相比，其编码区出现了33 - 66个核苷酸替换，导致13 - 16个氨基酸变化（Chowdhury等人，《病毒学文献》148卷，2437 - 2448页，2003年）。在本研究中，我们发现低代CAV（BL - 5）和高代CAV（BL - 5/P90）仅相差15个核苷酸替换，导致11个氨基酸变化。基于VP1的系统发育分析还表明，这两种分离株彼此接近，但与来自马来西亚的其他CAV分离株，即SMSC - 1和3 - 1并不接近。