Pławińska J, Jakubowski T, Rzewuska M, Binek M
Department of Preclinical Sciences, Faculty of Veterinary Medicine, Agricultural University, Ciszewskiego 8, 02-786 Warsaw, Poland.
Pol J Vet Sci. 2004;7(3):203-6.
Principal aim of this study was to examine fecal samples from pigs suffering from diarrhea for the presence of Lawsonia intracellularis, Brachyspira hyodysenteriae and Brachyspira pilosicoli. The molecular techniques such as PCR and nested PCR were employed to detect the presence of p78 fragment of genomic DNA specific for Lawsonia intracellularis as well as fragment of tlyA gene specific for Brachyspira hyodysenteriae and 16S rDNA gene of Brachyspira pilosicoli. We assumed that about 25% of pigs were infected with Lawsonia intracellularis, about 10% with Brachyspira hyodysenteriae and only 0,8% with Brachyspira pilosicoli. In about 3% mixed infection with L. intracellularis and B. hyodysenteriae was observed. Results were comparable in herds that differed in quantity, breeding technology, hygienic standards and preventive treatment with different chemotherapeutics.
本研究的主要目的是检查腹泻猪的粪便样本中是否存在胞内劳森菌、猪痢疾短螺旋体和毛样短螺旋体。采用PCR和巢式PCR等分子技术检测胞内劳森菌基因组DNA特异性p78片段以及猪痢疾短螺旋体特异性tlyA基因片段和毛样短螺旋体16S rDNA基因。我们假设约25%的猪感染了胞内劳森菌，约10%感染了猪痢疾短螺旋体，仅0.8%感染了毛样短螺旋体。约3%的猪出现了胞内劳森菌和猪痢疾短螺旋体的混合感染。在数量、养殖技术、卫生标准以及使用不同化学治疗剂进行预防性治疗方面存在差异的猪群中，结果具有可比性。
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