Melilli C, Barbano D M, Caccamo M, Calvo M A, Schembari G, Licitra G
CoRFiLaC, Regione Siciliana, 97100 Ragusa, Italy.
J Dairy Sci. 2004 Nov;87(11):3648-57. doi: 10.3168/jds.S0022-0302(04)73503-1.
Thirty-one 3.8-kg blocks of Ragusano cheese were made on each of 6 d starting with a different batch of raw milk on each day. On d 1, 3, and 5, cheeses were not presalted and on d 2, 4, and 6, all cheeses were presalted. Before brine salting, one of the 31 blocks of cheese was selected at random for analysis (i.e., at d 0). The remaining 30 blocks were randomly divided into 2 batches of 15 blocks each, one group was placed in 18% brine, and the other group was placed in saturated brine. For the 15 blocks within each of the 2 brine concentrations, 5 blocks each were placed in brine tanks at 12, 15, and 18 degrees C. Cheese blocks were sampled immediately before brine salting (d 0) and after 1, 4, 8, 16, and 24 d of brine salting. Presalting the curd with 2% added salt before stretching reduced the coliform count in the cheese by 1.41 log and resulted in a major reduction in early gas formation. Across all treatments in the present study, the average reduction in gas formation due to presalting was 75%. Reducing brine temperature had the second largest impact on reducing gas production, but did not reduce the coliform count in the cheese. Reducing brine temperature from 18 to 12 degrees C made a larger reduction in early gas formation in cheeses that were not presalted (from 6.8 to 1.8% gas holes, respectively) than in cheeses that were presalted (from 1.9 to 0.5% gas holes, respectively). To achieve the same absolute level of gas production in the nonpre-salted cheese as was achieved in presalted cheese in combination with 18 degrees C brine, the brine temperature for the nonpresalted cheese had to be lowered from 18 to 12 degrees C. Reducing brine concentration, although effective at increasing the rate of salt penetration into the block, did not have any impact on coliform count and had minimal impact on reducing gas production. The condition where reducing brine concentration was able to make a reduction in gas production was for cheeses that were not presalted and brined at 18 degrees C. Presalting is a very simple and practical approach to reducing the problem of early gas formation in combination with strategies to improve milk quality and cheese making conditions. Further work is needed to understand the impact of different levels of presalting on death of coliforms and gas production in the cheese.
在6天中的每一天,都用不同批次的原料乳制作了31块3.8千克的拉古萨诺奶酪。在第1、3和5天,奶酪不进行预加盐处理;在第2、4和6天,所有奶酪都进行预加盐处理。在盐水腌制前,从31块奶酪中随机选取一块进行分析(即第0天)。其余30块奶酪随机分为两组,每组15块,一组放入18%的盐水中,另一组放入饱和盐水中。对于两种盐水浓度下的15块奶酪,每种浓度各有5块分别置于12、15和18摄氏度的盐水槽中。在盐水腌制前(第0天)以及腌制1、4、8、16和24天后对奶酪块进行采样。在拉伸前用添加2%的盐对凝乳进行预腌制,可使奶酪中的大肠菌群数量减少1.41个对数级,并显著减少早期气体的产生。在本研究的所有处理中,预腌制导致的气体产生平均减少了75%。降低盐水温度对减少气体产生的影响次之,但并未降低奶酪中的大肠菌群数量。将盐水温度从18摄氏度降至12摄氏度,对未预加盐的奶酪早期气体产生的减少幅度更大(分别从6.8%降至1.8%的气孔率),比对预加盐的奶酪(分别从1.9%降至0.5%的气孔率)更大。为了使未预加盐的奶酪达到与预加盐奶酪在18摄氏度盐水条件下相同的绝对气体产生水平,未预加盐奶酪的盐水温度必须从18摄氏度降至12摄氏度。降低盐水浓度虽然能有效提高盐分渗入奶酪块的速率,但对大肠菌群数量没有影响,对减少气体产生的影响也微乎其微。降低盐水浓度能够减少气体产生的情况是针对未预加盐且在18摄氏度下腌制的奶酪。预腌制是一种非常简单且实用的方法,可结合提高牛奶质量和奶酪制作条件的策略来减少早期气体产生的问题。需要进一步开展工作来了解不同预腌制水平对奶酪中大肠菌群死亡和气体产生的影响。