Wang Xiao-hua, Wei Ya-ming, Bai Hai, Ou Jian-feng, Lu Ji-hong, Zheng Rong-liang
Department of Biochemistry and Molecular Biology, Guangzhou Medical College, Guangzhou 510182, China.
Di Yi Jun Yi Da Xue Xue Bao. 2004 Oct;24(10):1160-3.
To investigate the mechanisms underlying the effect of selenium dioxide (SeO(2)) on the proliferation, apoptosis, and apoptosis-related gene expressions of Bcl-2 and p53 in 3 leukemia cell lines NB4, K562 and HL-60.
The three leukemia cell lines were treated with 3, 10 and 30 mmol/L SeO(2) and apoptosis detected by flow cytometry and analysis of p53 and Bcl-2 expressions.
SeO(2) at 10 and 30 mmol/L could inhibit the proliferation of three leukemia cell lines. SeO(2) treatment at 30 mmol/L for 48 h induced an apoptosis rate of 54.0 %, 46.5 %, 49.6 % in NB4, K562, and HL-60 cells respectively, and down-regulated Bcl-2 expression in NB4 and K562 but not in HL-60 cells.
SeO(2) can induce apoptosis in NB4, K562 and HL-60 leukemia cells, involving the down-regulation of Bcl-2 and up-regulation of p53.
探讨二氧化硒（SeO₂）对3种白血病细胞系NB4、K562和HL-60增殖、凋亡及凋亡相关基因Bcl-2和p53表达的影响机制。
用3、10和30 mmol/L的SeO₂处理这3种白血病细胞系，通过流式细胞术检测凋亡情况，并分析p53和Bcl-2的表达。
10和30 mmol/L的SeO₂可抑制3种白血病细胞系的增殖。30 mmol/L的SeO₂处理48小时后，NB4、K562和HL-60细胞的凋亡率分别为54.0%、46.5%、49.6%，且下调了NB4和K562细胞中Bcl-2的表达，但未下调HL-60细胞中Bcl-2的表达。
SeO₂可诱导NB4、K562和HL-60白血病细胞凋亡，这涉及Bcl-2的下调和p53的上调。
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