成像可卡因引起的清醒大鼠中脑皮质边缘多巴胺能系统的变化。
Imaging cocaine-induced changes in the mesocorticolimbic dopaminergic system of conscious rats.
作者信息
Febo Marcelo, Segarra Annabell C, Tenney Jeffrey R, Brevard Mathew E, Duong Timothy Q, Ferris Craig F
机构信息
Center for Comparative NeuroImaging, University of Massachusetts Medical School, CCNI Building, 303 Belmont Street, Worcester, MA 01655, USA.
出版信息
J Neurosci Methods. 2004 Oct 30;139(2):167-76. doi: 10.1016/j.jneumeth.2004.04.028.
Functional magnetic resonance imaging (fMRI) was used to assess the effects of cocaine on brain activation in fully conscious rats. Methods were developed to image cocaine-induced changes in blood-oxygen-level-dependent (BOLD) signal without the peripheral cardiac and respiratory complications associated with psychostimulant administration. Using spin echo planar imaging (EPI), conscious rats were imaged in a 4.7 T spectrometer prior to and following the intracerebroventricular injection of cocaine (20 microg) in artificial cerebrospinal fluid (10 uL). Within 5 min of injection, there was a significant increase in BOLD signal intensity in the substantia nigra, ventral tegmental area, nucleus accumbens, dorsal striatum and prefrontal cortex, as compared to vehicle controls. Minimal negative BOLD signal changes were observed in response to cocaine and no significant perturbations in normal cardiovascular and respiratory function. These findings demonstrate the technical feasibility of studying psychostimulant-induced brain activity using functional MRI in conscious rats.
功能磁共振成像（fMRI）被用于评估可卡因对完全清醒大鼠大脑激活的影响。已开发出一些方法来成像可卡因诱导的血氧水平依赖（BOLD）信号变化，同时避免与精神兴奋剂给药相关的外周心脏和呼吸并发症。使用自旋回波平面成像（EPI），在向清醒大鼠脑室内注射可卡因（20微克）于人工脑脊液（10微升）之前和之后，在4.7T光谱仪中对其进行成像。注射后5分钟内，与溶剂对照组相比，黑质、腹侧被盖区、伏隔核、背侧纹状体和前额叶皮质的BOLD信号强度显著增加。对可卡因的反应观察到最小的负性BOLD信号变化，并且正常心血管和呼吸功能没有明显扰动。这些发现证明了在清醒大鼠中使用功能磁共振成像研究精神兴奋剂诱导的大脑活动的技术可行性。
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