1997年至2002年间特拉凯亚大学医院布鲁氏菌分离株的菌种和生物变种分布情况

[Species and biovar distribution of Brucella isolates in Trakya University Hospital between 1997-2002].

作者信息

Kuloğlu Figen, Erdenliğ Sevil, Akata Filiz, Tansel Ozlem, Gürcan Saban, Tuğrul H Murat

机构信息

Trakya Universitesi Tip Fakültesi, Klinik Bakteriyoloji ve infeksiyon Hastaliklari Anabilim Dali, Edirne.

出版信息

Mikrobiyol Bul. 2004 Jul;38(3):187-91.

PMID:15490837

Abstract

The aim of this study was to evaluate the species and biovar distribution of Brucella spp. isolated from blood cultures in Clinical Microbiology Laboratory of Trakya University Hospital, between 1997-2002. A total of 48 Brucella spp. have been isolated from 14.815 patients (0.3%), and the strains were identified according to CO2 requirement, H2S production, basic fuchsin and thionin sensitivity, lysis by Tbilisi phages, and presence of agglutination with monospecific A and M antisera. As a result, 47 (97.9%) isolates were identified as B. melitensis, and one as B. abortus (2.1%). Forty two (89.4%) of B. melitensis isolates were biovar 3, and five (10.6%) were biovar 1, while the single isolate of B. abortus was identified as an atypical strain.

摘要

本研究的目的是评估1997年至2002年间从特拉凯大学医院临床微生物实验室血培养中分离出的布鲁氏菌属的种类和生物变种分布。共从14815例患者（0.3%）中分离出48株布鲁氏菌属，根据二氧化碳需求、硫化氢产生、碱性品红和硫堇敏感性、第比利斯噬菌体裂解以及与单特异性A和M抗血清的凝集反应对菌株进行鉴定。结果，47株（97.9%）分离株被鉴定为羊种布鲁氏菌，1株（2.1%）为牛种布鲁氏菌。42株（89.4%）羊种布鲁氏菌分离株为生物变种3，5株（10.6%）为生物变种1，而单株牛种布鲁氏菌被鉴定为非典型菌株。