Mathioudaki Konstantina, Scorilas Andreas, Papadokostopoulou Alexandra, Xynopoulos Dimitrios, Arnogianaki Niki, Agnanti Niki, Talieri Maroulio
G. Papanicolaou Research Center of Oncology, Saint Savas Hospital, GR-11522 Athens, Greece.
Biol Chem. 2004 Sep;385(9):779-83. doi: 10.1515/BC.2004.101.
Apoptosis is an active process regulated by a variety of genes. Recently, the molecular cloning, physical mapping and expression analysis of a novel gene of the Bcl-2 family, BCL2L12, was reported. Expression analysis of the BCL2L12 gene in breast cancer confirmed an association of BCL2L12 with favorable prognosis of patients. In the present study, the expression of the BCL2L12 gene was analyzed in colon cancer by RT-PCR. Two transcripts, BCL2L12 and BCL2L12-A, were overexpressed in the cancer tissues as compared to their paired normal mucosa. An association was found between BCL2L12-A transcript expression and nodal status, as well as Dukes' stage. The BCL2L12-A transcript appears to be of importance for colon cancer since its expression is associated with disease progression.
细胞凋亡是一个由多种基因调控的活跃过程。最近，有报道称对Bcl-2家族的一个新基因BCL2L12进行了分子克隆、物理图谱绘制及表达分析。BCL2L12基因在乳腺癌中的表达分析证实其与患者的良好预后相关。在本研究中，通过逆转录聚合酶链反应（RT-PCR）分析了BCL2L12基因在结肠癌中的表达。与配对的正常黏膜相比，两种转录本BCL2L12和BCL2L12-A在癌组织中均过度表达。发现BCL2L12-A转录本表达与淋巴结状态以及Dukes分期之间存在关联。BCL2L12-A转录本似乎对结肠癌很重要，因为其表达与疾病进展相关。
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