ETS-1转录因子激活小鼠UDP-半乳糖：GA2/GM2/GD2/GT2半乳糖基转移酶基因的表达。

ETS-1 transcription factor activates the expression of mouse UDP-Gal:GA2/GM2/GD2/GT2 galactosyltransferase gene.

作者信息

Raimondi Lina P, Daniotti Jose L, Maccioni Hugo J F

机构信息

Departamento de Química Biológica, Facultad de Ciencias Químicas, Centro de Investigaciones en Química Biológica de Córdoba, CIQUIBIC (UNC-CONICET), Universidad Nacional de Córdoba, Ciudad Universitaria, 5000 Córdoba, Argentina.

出版信息

FEBS Lett. 2004 Oct 22;576(3):487-91. doi: 10.1016/j.febslet.2004.09.066.

DOI:10.1016/j.febslet.2004.09.066

PMID:15498585

Abstract

UDP-Gal:GA2/GM2/GD2/GT2 galactosyltransferase (Gal-T2) transfers galactose to the terminal N-acetylgalactosamine of either the neutral glycolipid GA2 or of the gangliosides GM2, GD2 and GT2. Previous studies revealed a tight regulation of Gal-T2 activity and mRNA expression during development of the rat CNS. Here, we study in PC12 cells the cis-acting elements involved in the activation of a fragment of 211 bp around the transcription initiation site of the mouse Gal-T2 promoter. Mutagenesis, competition experiments and functional assays showed that the Ets-1 transcription factor is involved in the activation of the Gal-T2 promoter.

摘要

UDP-半乳糖：GA2/GM2/GD2/GT2半乳糖基转移酶（Gal-T2）将半乳糖转移至中性糖脂GA2或神经节苷脂GM2、GD2和GT2的末端N-乙酰半乳糖胺上。先前的研究揭示了大鼠中枢神经系统发育过程中Gal-T2活性和mRNA表达受到严格调控。在此，我们在PC12细胞中研究了小鼠Gal-T2启动子转录起始位点周围211 bp片段激活过程中涉及的顺式作用元件。诱变、竞争实验和功能分析表明，Ets-1转录因子参与了Gal-T2启动子的激活。