妊娠足月时宫颈黏液栓中的基质金属蛋白酶及其抑制剂
Matrix metalloproteinases and their inhibitors in the cervical mucus plug at term of pregnancy.
作者信息
Becher Naja, Hein Merete, Danielsen Carl Christian, Uldbjerg Niels
机构信息
Department of Obstetrics and Gynecology, Skejby University Hospital, University of Aarhus, DK-8200 Aarhus N, Denmark.
出版信息
Am J Obstet Gynecol. 2004 Oct;191(4):1232-9. doi: 10.1016/j.ajog.2004.03.023.
OBJECTIVE
This study was undertaken to evaluate the presence of matrix metalloproteinases (MMPs) and their inhibitors in the cervical mucus plug obtained during active labor at term.
STUDY DESIGN
Cervical mucus plugs from 17 healthy women in normal active labor were homogenized, extracted, and analyzed by 4 different assays.
RESULTS
Gelatin zymography revealed large amounts of MMP-2 and MMP-9, and triple-helical collagen degradation demonstrated collagenase activity (MMP-8). Reverse zymography showed that tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) and TIMP-2 are detectable in the cervical mucus plug. Quantification by enzyme-linked immunosorbent assay confirmed the presence of MMP-2, MMP-8, MMP-9, and TIMP-1.
CONCLUSION
Our results suggest that proteolytic or nonproteolytic effects of MMP and TIMP may play a part in the function of the cervical mucus plug of pregnancy.
目的
本研究旨在评估足月活跃分娩时获得的宫颈黏液栓中基质金属蛋白酶（MMPs）及其抑制剂的存在情况。
研究设计
对17名正常活跃分娩的健康女性的宫颈黏液栓进行匀浆、提取，并通过4种不同的检测方法进行分析。
结果
明胶酶谱分析显示大量的MMP-2和MMP-9，三螺旋胶原降解显示出胶原酶活性（MMP-8）。反向酶谱分析表明在宫颈黏液栓中可检测到基质金属蛋白酶组织抑制剂1（TIMP-1）和TIMP-2。通过酶联免疫吸附测定进行定量证实了MMP-2、MMP-8、MMP-9和TIMP-1的存在。
结论
我们的结果表明，MMP和TIMP的蛋白水解或非蛋白水解作用可能在妊娠宫颈黏液栓的功能中起作用。
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