Krishnakumar Subramanian, Lakshmi Amirtha, Shanmugam Mahesh P, Vanitha K, Biswas Jyotirmay
Department of Ocular Pathology, Medical and Vision Research Foundations, Sankara Nethralaya, Chennai, Tamil Nadu, India.
Ocul Immunol Inflamm. 2004 Jun;12(2):127-35. doi: 10.1080/09273940490895335.
Nm23 is a metastasis-suppressor protein. Decreased nm23 protein contributes to aggressiveness in many tumors. Nm23 immunoreactivity was studied in retinoblastoma and correlated with differentiation and invasiveness.
Immunohistochemistry was performed on 73 formalin-fixed, paraffin-embedded specimens of retinoblastoma. Western blot was conducted to confirm the immunohistochemical study. Prognostic features such as differentiation, invasion of choroid, optic nerve, and orbit, and metastasis were analyzed.
Intense nm23 immunoreactivity was seen in 61% of the retinoblastomas with no invasion and faint nm23 immunoreactivity was seen in 85% of the retinoblastomas with invasion (p < 0.001). Poorly differentiated retinoblastoma showed decreased nm23 immunoreactivity compared to well-differentiated retinoblastomas (p = 0.02). An inverse correlation was observed between invasion of choroid, optic nerve, orbit, and metastasis, and nm23 immunoreactivity. Western blot assays of fresh tumor extracts confirmed the immunohistochemical findings.
Decreased nm23 immunoreactivity was seen in poorly differentiated retinoblastomas and in retinoblastomas with invasiveness. These findings may lay the groundwork for further studies to better understand the molecular mechanisms and provide a more accurate prediction of invasion and metastasis of retinoblastoma.
Nm23是一种转移抑制蛋白。Nm23蛋白水平降低与多种肿瘤的侵袭性有关。本研究对视网膜母细胞瘤中的Nm23免疫反应性进行了研究，并将其与肿瘤的分化程度和侵袭性相关联。
对73例福尔马林固定、石蜡包埋的视网膜母细胞瘤标本进行免疫组织化学检测。采用蛋白质免疫印迹法对免疫组织化学研究结果进行验证。分析肿瘤的分化程度、脉络膜、视神经和眼眶侵袭情况以及转移等预后特征。
61%无侵袭的视网膜母细胞瘤可见强烈的Nm23免疫反应性，而85%有侵袭的视网膜母细胞瘤可见微弱的Nm23免疫反应性（p < 0.001）。与高分化视网膜母细胞瘤相比，低分化视网膜母细胞瘤的Nm23免疫反应性降低（p = 0.02）。脉络膜、视神经、眼眶侵袭及转移与Nm23免疫反应性呈负相关。新鲜肿瘤提取物的蛋白质免疫印迹分析证实了免疫组织化学研究结果。
低分化视网膜母细胞瘤及具有侵袭性的视网膜母细胞瘤中Nm23免疫反应性降低。这些发现可能为进一步研究视网膜母细胞瘤的分子机制奠定基础，并为更准确地预测视网膜母细胞瘤的侵袭和转移提供依据。
