High efficiency DNA mutagenesis mediated by using in vitro transcription, DNase I digestion, and RT-PCR.

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作者信息

Xin Wen, Huang Da-Wei, Xiao Hui

机构信息

Chinese Academy of Sciences, Beijing, P.R. China.

出版信息

Biotechniques. 2004 Oct;37(4):556, 558, 560. doi: 10.2144/04374BM06.

DOI:10.2144/04374BM06

PMID:15517966

Abstract

摘要

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相似文献

High efficiency DNA mutagenesis mediated by using in vitro transcription, DNase I digestion, and RT-PCR.

Biotechniques. 2004 Oct;37(4):556, 558, 560. doi: 10.2144/04374BM06.

Use of manganese in RT-PCR eliminates PCR artifacts resulting from DNase I digestion.

Biotechniques. 1997 Jun;22(6):1128-32. doi: 10.2144/97226st05.

Quantitative RT-PCR amplification of RNA in single mouse oocytes and preimplantation embryos.

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Optimization of Dnase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR.

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