重症肌无力中的胸腺细胞：胸腺和胸腺瘤中淋巴细胞的双色流式细胞术分析

Thymus cells in myasthenia gravis: a two-colour flow cytometric analysis of lymphocytes in the thymus and thymoma.

作者信息

Fujii Y, Hayakawa M, Nakahara K

机构信息

First Department of Surgery, Osaka University Medical School, Japan.

出版信息

J Neurol. 1992 Feb;239(2):82-8. doi: 10.1007/BF00862978.

DOI:10.1007/BF00862978

PMID:1552308

Abstract

The thymus in patients with myasthenia gravis has germinal centres and the thymic lymphocytes spontaneously produce anti-acetylcholine receptor antibody in vitro. This suggests indirectly that the myasthenic thymus contains mature T and B cells that are autoreactive. We report a two colour flow cytometric analysis of the surface antigen phenotype of lymphocytes in the thymus and in thymoma in 24 patients and in 14 normal thymuses. The results show a decline in the proportion of double positive immature thymocytes with age. This process seems to be partially disrupted in patients with myasthenia gravis. In addition titres of anti-acetylcholine receptor antibody were positively correlated with the proportion of mature single positive T cells and non-T DR positive cells.

摘要

重症肌无力患者的胸腺有生发中心，胸腺淋巴细胞在体外可自发产生抗乙酰胆碱受体抗体。这间接提示重症肌无力患者的胸腺含有自身反应性的成熟T细胞和B细胞。我们报告了对24例患者及14个正常胸腺的胸腺和胸腺瘤中淋巴细胞表面抗原表型进行的双色流式细胞术分析。结果显示，双阳性未成熟胸腺细胞的比例随年龄下降。这一过程在重症肌无力患者中似乎部分受到破坏。此外，抗乙酰胆碱受体抗体的滴度与成熟单阳性T细胞和非T DR阳性细胞的比例呈正相关。