来自颊黏膜上皮细胞的DNA全基因组扩增的基因分型准确性。

Genotyping accuracy for whole-genome amplification of DNA from buccal epithelial cells.

作者信息

Leviel Keren, Olarte Megan, Sullivan Patrick F

机构信息

Department of Genetics, University of North Carolina at Chapel Hill, 27599-7264, USA.

出版信息

Twin Res. 2004 Oct;7(5):482-4. doi: 10.1375/1369052042335214.

DOI:10.1375/1369052042335214

PMID:15527664

Abstract

We compared the accuracy of genotyping for DNA extracted from lymphocytes to that of DNA amplified from buccal epithelial cells. Amplification was via a rolling circle/phi29 DNA polymerase commercial kit. Paired buccal and lymphocyte DNA samples were available from 30 individuals. All samples were genotyped for 12 SNPs, 5 microsatellites and 2 VNTRs. The accuracy of genotyping (no-call proportions, reproducibility, and concordance) was similar for DNA from lymphocytes in comparison to amplified DNA from buccal samples. If used with caution, these data suggest that rolling-circle whole-genome amplification can be used to increase the DNA mass available for large-scale genotyping projects based on DNA from buccal cells.

摘要

我们比较了从淋巴细胞中提取的DNA基因分型准确性与从颊上皮细胞中扩增的DNA的基因分型准确性。扩增通过滚环/phi29 DNA聚合酶商业试剂盒进行。从30名个体中获得了配对的颊部和淋巴细胞DNA样本。所有样本均针对12个单核苷酸多态性（SNP）、5个微卫星和2个可变数目串联重复序列（VNTR）进行基因分型。与颊部样本扩增的DNA相比，淋巴细胞DNA的基因分型准确性（无调用比例、重复性和一致性）相似。如果谨慎使用，这些数据表明滚环全基因组扩增可用于增加基于颊细胞DNA的大规模基因分型项目可用的DNA量。