用于毛细管高效液相色谱-纳米电喷雾质谱联用的纳流梯度发生器

Nanoflow gradient generator for capillary high-performance liquid chromatography-nanoelectrospray mass spectrometry.

作者信息

Ito Shinya, Yoshioka Shinji, Ogata Izumi, Takeda Akihiro, Yamashitaa Eri, Deguchi Kisaburo

机构信息

Hitachi High-Technologies Co., Hitachinaka, Japan.

出版信息

J Chromatogr A. 2004 Oct 8;1051(1-2):19-23. doi: 10.1016/j.chroma.2004.05.006.

DOI:10.1016/j.chroma.2004.05.006

PMID:15532551

Abstract

A novel nanoflow gradient generator using a 10-port switching valve with two injection loops installed, which is referred to here as the "Asymptotic-Trace-10-Port-Valve" (AT10PV) nanoGR generator, has been applied to capillary high-performance liquid chromatography (HPLC)-microelectrospray (microESI) or nanoelectrospray (nanoESI) time-of-flight mass spectrometry (TOF MS). In this study, performance of this capillary HPLC-micro/nanoESI-MS system was tested at a flow rate of 200 nl/min by using three typical peptides (angiotensins I, II, and III: 50 fmol each). The result demonstrated that this system provides reasonably good repeatability of peak retention times (R. S.D. of less than 0.5%). Sequential runs of a series of sample injections were performed in the same manner as conventional analysis at microflow rates.

摘要

一种新型的纳流梯度发生器，它使用安装了两个进样环的十通切换阀，在此称为“渐近追踪十通阀”（AT10PV）纳流梯度发生器，已应用于毛细管高效液相色谱（HPLC）-微电喷雾（microESI）或纳电喷雾（nanoESI）飞行时间质谱（TOF MS）。在本研究中，使用三种典型肽（血管紧张素I、II和III：每种50 fmol），以200 nl/min的流速测试了该毛细管HPLC-微/纳ESI-MS系统的性能。结果表明，该系统提供了相当好的峰保留时间重复性（相对标准偏差小于0.5%）。一系列样品进样的连续运行以与微流速常规分析相同的方式进行。